Comparison of karyotype and rDNA-distribution in somatic chromosomes of Bowenia species (Stangeriaceae, Cycadales)

Kokubugata, G., Kondo, K., Wilson, G.W., Randall, L.M., van der Schans, A., and Morris, D.K. (2000) Comparison of karyotype and rDNA-distribution in somatic chromosomes of Bowenia species (Stangeriaceae, Cycadales). Australian Systematic Botany, 13 (1). pp. 15-20.

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DOI: 10.1071/SB98028

View at Publisher Website: http://dx.doi.org/10.1071/SB98028

Abstract

Somatic chromosomes at mitotic metaphase of Bowenia serrulata, B. spectabilis and B. sp. 'Tinaroo' is investigated by the standard aceto-orcein staining method and the fluorescent in situ hybridisation method (FISH) with ribosomal DNA (rDNA) probe. Bowenia serrulata, B. spectabilis and B. sp. 'Tinaroo' each have a chromosome number of 2n = 18. The karyotype of B. serrulata exhibits 10 median-centromeric chromosomes, while B. spectabilis and B. sp. 'Tinaroo' exhibit eight median-centromeric chromosomes. By using FISH, B. serrulata, B. spectabilis and B. sp. 'Tinaroo' show a hybridisation signal on the satellite of the short arm of two submedian-centromeric chromosomes. However, the other hybridisation signal pattern is different among B. serrulata, B. spectabilis and B. sp. 'Tinaroo'.


Item ID:	24598
Item Type:	Article (Research - C1)
ISSN:	1446-5701
Date Deposited:	05 Feb 2013 23:37
FoR Codes:	06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060309 Phylogeny and Comparative Analysis @ 50% 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 50%
SEO Codes:	96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960806 Forest and Woodlands Flora, Fauna and Biodiversity @ 100%
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