Development of ten polymorphic microsatellite loci for the sea snake Hydrophis elegans (Elapidae: Hydrophiinae) and cross-species amplification for fifteen marine hydrophiine species

Lukoschek, Vimoksalehi, and Avise, John C. (2011) Development of ten polymorphic microsatellite loci for the sea snake Hydrophis elegans (Elapidae: Hydrophiinae) and cross-species amplification for fifteen marine hydrophiine species. Conservation Genetics Resources, 3 (3). pp. 497-501.

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DOI: 10.1007/s12686-011-9388-5

View at Publisher Website: http://dx.doi.org/10.1007/s12686-011-938...

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Abstract

We developed ten microsatellite loci for the elegant sea snake, Hydrophis elegans, from partial genomic DNA libraries using a repeat enrichment protocol. Eight loci had nine or more alleles per locus (maximum 20), while the other two had three and seven. All ten loci amplified successfully in 11 of the 15 additional hydrophiine sea snake species screened. Nine loci amplified successfully for three species and eight amplified successfully for the remaining species. Based on this highly successful cross-amplification we expect these ten loci to be useful markers for investigating population genetic structure, gene flow and parentage for all sea snake species from the Hydrophis group.


Item ID:	18377
Item Type:	Article (Research - C1)
ISSN:	1877-7260
Keywords:	microsatellite loci; hydrophiinae; hydrophis; sea snakes; connectivity; parentage
Additional Information:	This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Date Deposited:	19 Sep 2011 22:49
FoR Codes:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 70% 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 30%
SEO Codes:	97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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