Isolation and characterization of eight microsatellite loci from silver-lipped pearl oyster Pinctada maxima

Smith, C., Benzie, J.A.H, and Wilson, K.J. (2003) Isolation and characterization of eight microsatellite loci from silver-lipped pearl oyster Pinctada maxima. Molecular Ecology Notes, 3 (1). pp. 125-127.

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Abstract

We have developed di- and tetra-nucleotide microsatellite markers for the silver-lipped pearl oyster Pinctada maxima. Screening of approximately 50 000 clones resulted in the identification of 74 microsatellites. Fluorescent PCR was optimized for eight polymorphic loci. In a sample of 1637 individuals, these eight loci possessed between 14 and 68 alleles (average 29.8), with observed and expected heterozygosity ranging from 0.479 to 0.891 and 0.872 to 0.972, respectively.

Item ID: 10094
Item Type: Article (Research - C1)
ISSN: 1755-0998
Keywords: microsatellites; pearl oyster; Pinctada maxima; population genetics; marine science
Date Deposited: 12 Apr 2010 02:03
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830104 Aquaculture Oysters @ 50%
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