Isolation and characterization of eight microsatellite loci from silver-lipped pearl oyster Pinctada maxima
Smith, C., Benzie, J.A.H, and Wilson, K.J. (2003) Isolation and characterization of eight microsatellite loci from silver-lipped pearl oyster Pinctada maxima. Molecular Ecology Notes, 3 (1). pp. 125-127.
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Abstract
We have developed di- and tetra-nucleotide microsatellite markers for the silver-lipped pearl oyster Pinctada maxima. Screening of approximately 50 000 clones resulted in the identification of 74 microsatellites. Fluorescent PCR was optimized for eight polymorphic loci. In a sample of 1637 individuals, these eight loci possessed between 14 and 68 alleles (average 29.8), with observed and expected heterozygosity ranging from 0.479 to 0.891 and 0.872 to 0.972, respectively.
| Item ID: | 10094 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1755-0998 |
| Keywords: | microsatellites; pearl oyster; Pinctada maxima; population genetics; marine science |
| Date Deposited: | 12 Apr 2010 02:03 |
| FoR Codes: | 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 100% |
| SEO Codes: | 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50% 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830104 Aquaculture Oysters @ 50% |
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