A robust fluorescent glycerol kinase biosensor for reliable quantification of glycerol
Sorenson, Alanna E., and Schaeffer, Patrick M. (2027) A robust fluorescent glycerol kinase biosensor for reliable quantification of glycerol. Talanta, 312. 130327.
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Abstract
Glycerol is a natural component of many biological samples, beverages and foodstuffs making accurate quantification relevant to a wide variety of industries and applications. Here we present a simple, rapid, and reliable biosensor for quantification of glycerol in a variety of sample types including water, alcoholic beverages, balsamic vinegar, honey, syrup and artificial human urine. This system leverages GFP-tagged Escherichia coli glycerol kinase with differential scanning fluorimetry of GFP-tagged proteins for direct quantification of glycerol via binding-induced increases in thermal stability. The GFP tag enables additional indication of potential matrix effects. The biosensor has a broad linear range of 31 μM – 7.5 mM and a limit of detection of 19 μM. Good agreement was obtained between the fluorescent glycerol kinase biosensor and a commercial glycerol quantification kit with glycerol-spiked artificial urine as well as a variety of alcoholic beverages and foodstuffs.
| Item ID: | 93422 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1873-3573 |
| Copyright Information: | © 2026 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Date Deposited: | 07 Aug 2026 03:53 |
| FoR Codes: | 34 CHEMICAL SCIENCES > 3401 Analytical chemistry > 340108 Sensor technology (incl. chemical aspects) @ 50% 34 CHEMICAL SCIENCES > 3401 Analytical chemistry > 340102 Bioassays @ 50% |
| SEO Codes: | 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences @ 50% 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280103 Expanding knowledge in the biomedical and clinical sciences @ 50% |
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