Sean, Afifah, Lim, Tzer Shyun, Domingos, Jose A., Uichanco, Joseph A., Shen, Xueyan, and Gibson-Kueh, Susan (2025) Air Nanobubbles Enhance Viable Bacteria Counts, Abundance of Nitrifying Bacteria, and Reduce Nitrite Levels in Marine Recirculation Aquaculture Systems. Fishes, 10 (11). 550. pp. 1-16.

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Abstract

Recirculating aquaculture systems (RAS) address pollution, disease, and sustainability in commercial fish farming, but marine RAS are limited by biofilter maturation and nitrification. This study investigated the effects of air nanobubbles on water quality, fish growth, and bacterial communities in marine RAS stocked with juvenile Malabar red snapper, barramundi and saline-tolerant hybrid tilapia. Flow cytometry was evaluated as a rapid management tool for non-culturable microbes, finding viable bacterial counts 30–100 times higher than conventional total plate counts. There were no significant differences in fish growth, survival, or Feed Conversion Ratio between groups, likely due to low stocking densities (<20 kg/m³) and high water exchange rates (>100%/hour), indicating low system stress. Air nanobubbles did not significantly increase dissolved oxygen levels. While bacterial abundance in water was consistently higher in nanobubble-treated RAS (RAS-N), tank walls showed less biofilm. RAS-N also exhibited a higher abundance of nitrifying bacteria like Nitrospira and Marinobacter, leading to improved nitrogenous waste breakdown and lower nitrite levels. Future research should investigate nanobubbles’ benefits at higher stocking densities and longer durations to fully assess their impact on intensive aquaculture.

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