Characterisation of Lates calcarifer primary myoblast proliferation and differentiation from direct cell isolation
Trace, Craig, Wankell, Miriam, McFarlane, Craig, and Hebbard, Lionel (2026) Characterisation of Lates calcarifer primary myoblast proliferation and differentiation from direct cell isolation. In Vitro Cellular and Developmental Biology: animal. (In Press)
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Abstract
Cell type characterisation is pivotal for scientific research and discovery. In the literature, multiple fish cell types have been investigated. Nonetheless, primary myoblasts from barramundi (Lates calcarifer) remain poorly characterised, despite their potential applications for cellular aquaculture and disease modelling. This study characterised primary barramundi myoblasts by assessing their proliferative capacity and differentiation potential, using molecular and cellular biology techniques. The results show that isolated primary barramundi myoblasts exhibit a faster doubling time compared to previously characterised embryonic cell lines, and retain the ability to form mature myotubes, even in cultures containing mixed cell populations. This study presents the first detailed description of primary barramundi myoblasts, providing foundational data to support the future use of these cells.
| Item ID: | 91369 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1543-706X |
| Keywords: | Myoblast; MyoD1; Myogenin; Differentiation; Myotube Formation; Barramundi |
| Copyright Information: | © The Society for In Vitro Biology 2026 |
| Funders: | Australian Government Department of Education (DoE) |
| Projects and Grants: | DoE Australian Economic Accelerator Ignite Grant |
| Date Deposited: | 11 May 2026 00:25 |
| FoR Codes: | 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310102 Cell development, proliferation and death @ 80% 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3001 Agricultural biotechnology > 300199 Agricultural biotechnology not elsewhere classified @ 20% |
| SEO Codes: | 24 MANUFACTURING > 2499 Other manufacturing > 249999 Other manufacturing not elsewhere classified @ 50% 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1099 Other animal production and animal primary products > 109999 Other animal production and animal primary products not elsewhere classified @ 50% |
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