Development of nine microsatellite markers for Pomacentrus amboinensis

Jones, David, Jerry, Dean, McCormick, Mark, and Bay, Line (2008) Development of nine microsatellite markers for Pomacentrus amboinensis. Molecular Ecology Resources, 8 (6). pp. 1332-1334.

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Abstract

The relatively long pelagic larval duration of Pomacentrus amboinensis, a tropical fish, suggests the potential for long-distance dispersal; however, several nongenetic studies have found substantial self-recruitment at one location. To analyse patterns of connectivity of this species, primers for nine independent microsatellite loci were developed for P. amboinensis using a magnetic bead enrichment protocol. Twenty individuals from one location were analysed and observed heterozygosities ranged from 0.7 to 0.95. Eight of nine loci were in Hardy–Weinberg equilibrium and no evidence of linkage or null alleles were found.

Item ID: 8683
Item Type: Article (Refereed Research - C1)
Keywords: connectivity; microsatellite primers; Pomacentrus amboinensis; tropical fish; OIRS; marine sciences
ISSN: 1755-0998
Funders: ARC
Date Deposited: 02 Mar 2010 01:55
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070405 Fish Physiology and Genetics @ 50%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%
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