Liu, Qing, Sui, Zengying, Feng, Nuan, Huang, Yuhao, Li, Yonghong, Ahmed, Ishfaq, Ruethers, Thimo, Liang, Hui, Li, Zhenxing, Lopata, Andreas L., and Sun, Lirui (2024) Characterization, Epitope Confirmation, and Cross-Reactivity Analysis of Parvalbumin from Lateolabrax maculatus by Multiomics Technologies. Journal of Agricultural and Food Chemistry, 72 (36). pp. 20077-20090.

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Abstract

Spotted seabass (Lateolabrax maculatus) is the second largest maricultural fish species in China and is the main trigger of food-related allergic reactions. Nevertheless, studies on the allergens of L. maculatus are limited. This study aimed to characterize pan-allergen parvalbumin from L. maculatus. Two proteins of about 11 kDa were purified and confirmed as parvalbumins by mass spectrometry. The IgG- and IgE-binding activities were evaluated through an immunoblotting assay. The molecular characteristics of β-parvalbumin were investigated by combining proteomics, genomics, and immunoinformatics approaches. The results indicated that β-parvalbumin consists of 109 amino acids with a molecular weight of 11.5 kDa and is the major allergen displaying strong IgE-binding capacity. In silico analysis and a dot blotting assay confirmed seven linear B cell epitopes distributed mainly on α-helixes and the calcium-binding loops. In addition, the cross-reactivity among 26 commonly consumed fish species was analyzed. The in-house generated anti-L. maculatus parvalbumin polyclonal antibody recognized 100% of the 26 fish species, demonstrating cross-reactivity and better binding capacity than the anticod parvalbumin antibody. Together, this study provides an efficient protocol to characterize allergens with multiomics methods and supports parvalbumin from L. maculatus as a candidate for fish allergen determination and allergy diagnosis.

Item ID:	83953
Item Type:	Article (Research - C1)
ISSN:	1520-5118
Copyright Information:	© 2024 American Chemical Society
Date Deposited:	30 Oct 2024 04:42
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3006 Food sciences > 300605 Food safety, traceability, certification and authenticity @ 30% 31 BIOLOGICAL SCIENCES > 3102 Bioinformatics and computational biology > 310205 Proteomics and metabolomics @ 20% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320401 Allergy @ 50%
SEO Codes:	20 HEALTH > 2001 Clinical health > 200101 Diagnosis of human diseases and conditions @ 50% 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1099 Other animal production and animal primary products > 109903 Fish product traceability and quality assurance @ 50%
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