Yasumaru, Fanny A., De Maesschalck, Celine, Wouters, Roeland, and Lemos, Daniel (2018) In vitro degree of protein hydrolysis using enzyme extracts from larval and postlarval Litopenaeus vannamei (Boone). Aquaculture Research, 49 (12). pp. 3933-3937.

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Abstract

[Extract] Live food is still widely used in shrimp hatcheries worldwide and total replacement with inert feed with similar results may only be feasible under laboratory conditions (Gallardo et al., 2002; Jones, Kamarudin, & Le Vay, 1993; Robinson, Samocha, Fox, Gandy, & McKee, 2005; Sheen & Huang, 1998). Additionally, technical details make the in vivo digestibility assessment not easy in larval and early postlarval shrimp. Thus, simple methods as in vitro degree of protein hydrolysis (DH) may be an option for first screening of feed ingredients (Lemos, Lawrence, & Siccardi, 2009; Tonheim, Nordgreen, Høgøy, Hamre, & Rønnestad, 2007). For example, in vitro pH-stat determination of the DH (%) quantifies the relative amount of peptide bonds cleaved in a protein substrate over a certain hydrolysis reaction with stable, physiological conditions. Correlation between in vitro DH and in vivo apparent protein digestibility has been reported for juvenile shrimp Litopenaeus vannamei (Lemos et al., 2009; Lemos, Carvalho, Yasumaru, & Tacon, 2015). Furthermore, the method has shown to be sensitive to feedstuff features impairing enzyme hydrolysis, for example, the presence of protease inhibitors, and type of processing (Ezquerra, Garcia-Carreño, & Haard, 1997; Galicia-González, Goytortúa-Bores, Palacios, & Civera-Cerecedo, 2010; Lemos, Córdova-Murueta, Navarrete del Toro, & Garcia-Carreño, 2004; Lemos et al., 2009). The present study aimed at determining in vitro protein digestion (DH) of a set of 10 feed ingredients using enzyme extracts from larval and postlarval shrimp.

Item ID:	82539
Item Type:	Article (Research - C1)
ISSN:	1365-2109
Keywords:	digestion; feedstuff; larval shrimp; pH-stat; postlarvae; protein hydrolysis
Copyright Information:	© 2018 John Wiley & Sons Ltd.
Date Deposited:	03 Apr 2024 23:55
FoR Codes:	30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 100%
SEO Codes:	10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1002 Fisheries - aquaculture > 100201 Aquaculture crustaceans (excl. rock lobster and prawns) @ 100%
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