Distinctive calcium isotopic composition of mice organs and fluids: implications for biological research

Cui, Meng Meng, Moynier, Frédéric, Su, Ben Xun, Dai, Wei, Mahan, Brandon, and Le Borgne, Marie (2023) Distinctive calcium isotopic composition of mice organs and fluids: implications for biological research. Analytical and Bioanalytical Chemistry, 415. pp. 6839-6850.

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The stable calcium (Ca) isotopes offer a minimally invasive method for assessing Ca balance in the body, providing a new avenue for research and clinical applications. In this study, we measured the Ca isotopic composition of soft tissues (brain, muscle, liver, and kidney), mineralized tissue (bone), and blood (plasma) from 10 mice (5 females and 5 males) with three different genetic backgrounds and same age (3 months old). The results reveal a distinctive Ca isotopic composition in different body compartments of mice, primally controlled by each compartment’s unique Ca metabolism and genetic background, independent of sex. The bones are enriched in the lighter Ca isotopes (δ44/40Cabone = − 0.10 ± 0.55 ‰) compared to blood and other soft tissues, reflecting the preferential incorporation of lighter Ca isotopes through bone formation, while heavier Ca isotopes remain preferentially in blood. The brain and muscle are enriched in lighter Ca isotopes (δ44/40Cabrain = − 0.10 ± 0.53 ‰; δ44/40Camuscle = 0.19 ± 0.41 ‰) relative to blood and other soft tissues, making the brain the isotopically lightest soft tissues of the mouse body. In contrast, the kidney is enriched in heavier isotopes (δ44/40Cakidney = 0.86 ± 0.31 ‰) reflecting filtration and reabsorption by the kidney. This study provides important insight into the Ca isotopic composition of various body compartments and fluids. Graphical Abstract: [Figure not available: see fulltext.]

Item ID: 80907
Item Type: Article (Research - C1)
ISSN: 1618-2650
Keywords: Body compartments, Calcium isotopes, Genetic background, Isotope fractionation, Sex
Copyright Information: © The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2023.
Date Deposited: 07 Feb 2024 00:44
FoR Codes: 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310101 Analytical biochemistry @ 100%
SEO Codes: 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences @ 100%
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