Commercial shellfish skin prick test extracts show critical variability in allergen repertoire

Ruethers, Thimo, Johnston, Elecia B., Karnaneedi, Shaymaviswanathan, Nie, Shuai, Nugraha, Roni, Taki, Aya C., Kamath, Sandip D., Williamson, Nicholas A., Mehr, Sam S., Campbell, Dianne E., and Lopata, Andreas L. (2023) Commercial shellfish skin prick test extracts show critical variability in allergen repertoire. Allergy. (In Press)

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Abstract

[Extract] Crustacean and mollusc (shellfish) allergy affects up to 3% of the general population, is usually lifelong and commonly triggers anaphylaxis.1 Allergen repertoire diversity among hundreds of edible shellfish species worldwide is poorly reflected in available in vivo and in vitro diagnostic tools for shellfish allergy. Skin prick testing (SPT) is often the preferred first-line diagnostic approach. However, widely utilized commercial SPT extracts are generally not standardized, limiting the diagnostic value of results.2 Asero et al. reported a heterogeneous abundance of three shellfish allergens in five commercial crustacean SPT extracts, resulting in 32 clinical profiles among 157 shrimp-allergic patients.3 In 2019, we demonstrated considerable variability in allergen repertoire and IgE-binding for 27 commercial fish SPT extracts.4 We now report an even greater, critical variability for 11 commercial crustacean and five mollusc SPT extracts, utilizing biochemical and immunological methods and mass spectrometry (see Appendix S1 for methodology and TableS1 for allergen extract details).

Item ID: 80370
Item Type: Article (Research - C1)
ISSN: 1398-9995
Keywords: allergy diagnosis, non-standardized allergen extracts, seafood allergens, shellfish allergy, tropomyosin
Copyright Information: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. © 2023 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.
Funders: National Health and Medical Research Council of Australia (NHMRC).
Projects and Grants: NHMRC GNT108665, NHMRC GNT113481, NHMRC GNT1124143
Date Deposited: 10 Oct 2023 23:33
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320401 Allergy @ 50%
32 BIOMEDICAL AND CLINICAL SCIENCES > 3206 Medical biotechnology > 320602 Medical biotechnology diagnostics (incl. biosensors) @ 50%
SEO Codes: 20 HEALTH > 2001 Clinical health > 200101 Diagnosis of human diseases and conditions @ 60%
20 HEALTH > 2004 Public health (excl. specific population health) > 200405 Food safety @ 10%
20 HEALTH > 2001 Clinical health > 200199 Clinical health not elsewhere classified @ 30%
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