Midbody-Localized Aquaporin Mediates Intercellular Lumen Expansion During Early Cleavage of an Invasive Freshwater Bivalve

Zieger, Elisabeth, Schwaha, Thomas, Burger, Katharina, Bergheim, Ina, Wanninger, Andreas, and Calcino, Andrew D. (2022) Midbody-Localized Aquaporin Mediates Intercellular Lumen Expansion During Early Cleavage of an Invasive Freshwater Bivalve. Frontiers in Cell and Developmental Biology, 10. 894434.

PDF (Published Version) - Published Version
Available under License Creative Commons Attribution.

Download (3MB) | Preview
View at Publisher Website: https://doi.org/10.3389/fcell.2022.89443...


Intercellular lumen formation is a crucial aspect of animal development and physiology that involves a complex interplay between the molecular and physical properties of the constituent cells. Embryos of the invasive freshwater mussel Dreissena rostriformis are ideal models for studying this process due to the large intercellular cavities that readily form during blastomere cleavage. Using this system, we show that recruitment of the transmembrane water channel protein aquaporin exclusively to the midbody of intercellular cytokinetic bridges is critical for lumenogenesis. The positioning of aquaporin-positive midbodies thereby influences the direction of cleavage cavity expansion. Notably, disrupting cytokinetic bridge microtubules impairs not only lumenogenesis but also cellular osmoregulation. Our findings reveal a simple mechanism that provides tight spatial and temporal control over the formation of luminal structures and likely plays an important role in water homeostasis during early cleavage stages of a freshwater invertebrate species.

Item ID: 80112
Item Type: Article (Research - C1)
ISSN: 2296-634X
Keywords: aquaporin; blastomere cleavage; freshwater invertebrate; lumenogenesis; midbody; osmoregulation
Copyright Information: Copyright © 2022 Zieger, Schwaha, Burger, Bergheim, Wanninger and Calcino. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Date Deposited: 30 Aug 2023 04:53
FoR Codes: 31 BIOLOGICAL SCIENCES > 3104 Evolutionary biology > 310499 Evolutionary biology not elsewhere classified @ 100%
SEO Codes: 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences @ 100%
Downloads: Total: 30
Last 12 Months: 5
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page