Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells
Wankell, Miriam, and Hebbard, Lionel (2023) Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells. In: Weiskirchen, Ralf, and Friedman, Scott, (eds.) Hepatic Stellate Cells. Methods in Molecular Biology, 2669 . Humana Press, New York, NY, USA, pp. 43-54.
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Abstract
The hepatic wound repair process involves cell types including healthy and injured hepatocytes, Kupffer and inflammatory cells, sinusoidal endothelial cells (SECs), and hepatic stellate cells (HSCs). Normally, in their quiescent state, HSCs are a reservoir for vitamin A, but in response to hepatic injury, they become activated myofibroblasts that play a key role in the hepatic fibrotic response. Activated HSCs express extracellular matrix (ECM) proteins, elicit anti-apoptotic responses, and proliferate, migrate, and invade hepatic tissues to protect hepatic lobules from damage. Extended liver injury can lead to fibrosis and cirrhosis, the deposition of ECM that is driven by HSCs. Here we describe in vitro assays that quantify activated HSC responses in the presence of inhibitors targeting hepatic fibrosis.
Item ID: | 78978 |
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Item Type: | Book Chapter (Scholarly Work) |
ISBN: | 978-1-0716-3207-9 |
ISSN: | 1940-6029 |
Keywords: | Apoptosis, HSCs, Invasion, Liver fibrosis, Migration, Proliferation |
Copyright Information: | © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023 |
Date Deposited: | 27 Jun 2023 01:52 |
FoR Codes: | 32 BIOMEDICAL AND CLINICAL SCIENCES > 3211 Oncology and carcinogenesis > 321101 Cancer cell biology @ 100% |
SEO Codes: | 20 HEALTH > 2001 Clinical health > 200105 Treatment of human diseases and conditions @ 100% |
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