Toxoplasma gondii profilin induces NLRP3 activation and IL-1β production/secretion in THP-1 cells

Pazoki, Hossein, Mirjalali, Hamed, Niyyati, Maryam, Seyed Tabaei, Seyed Javad, Mosaffa, Nariman, Shahrokh, Shabnam, Asadzadeh Ahdaei, Hamid, Kupz, Andreas, and Zali, Mohammad Reza (2023) Toxoplasma gondii profilin induces NLRP3 activation and IL-1β production/secretion in THP-1 cells. Microbial Pathogenesis, 180. 106120.

[img] PDF (Published) - Published Version
Restricted to Repository staff only

View at Publisher Website:


Toxoplasma gondii is a highly prevalent protozoan that infects a broad spectrum of warm-blooded animals. Profilin is a critical protein that plays a role in the movement and invasion of T. gondii. In the current study, we assessed how profilin stimulates inflammasomes and how it induces transcription and secretion of IL-1β. For this purpose, we assessed the level of TLR 2, 4, 5, and 9 expressions in a THP-1 cell line treated with profilin from T. gondii (TgP). In addition, we analyzed the expression levels of various inflammasomes, as well as IL-1β, and IL-18 in THP-1 cells treated with the NLRP3 inhibitor MCC950. TgP significantly increased the expression of TLR5 but the expression of TLR2, 4, and 9 was not significantly increased. In addition, TgP did not significantly increase the level of inflammasomes after 5 h. Treatment with MCC950 significantly reduced NLRP3 and IL-1β on both transcription and protein levels. Although the transcription level of NLRP3 was reduced 5 h after treatment with TgP, western blot analysis showed an increase in NLRP3. The western blot and ELISA analysis also showed that TgP increased both pro- and mature IL-1β. In summary, our study showed that NLRP3 most probably plays a pivotal role in the expression and production levels of IL-1β during the interaction between TgP and macrophages.

Item ID: 78915
Item Type: Article (Research - C1)
ISSN: 1096-1208
Keywords: IL-1β, NLRP3, Profilin, THP-1, TLR, Toxoplasma gondii
Copyright Information: © 2023 Elsevier Ltd. All rights reserved.
Date Deposited: 31 Oct 2023 02:18
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320704 Medical parasitology @ 100%
SEO Codes: 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280103 Expanding knowledge in the biomedical and clinical sciences @ 100%
Downloads: Total: 2
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page