Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver TRM cells
Valencia-Hernandez, Ana Maria, Zillinger, Thomas, Ge, Zhengyu, Tan, Peck S., Cozijnsen, Anton, Mcfadden, Geoffrey I., Lahoud, Mireille H., Caminsch, Irina, Barchet, Winfried, Heath, William R., and Fernandez-Ruiz, Daniel (2023) Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver TRM cells. Vaccine, 41 (5). pp. 1094-1107.
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Abstract
Tissue resident memory T cells (TRM cells) can provide effective tissue surveillance and can respond rapidly to infection. Vaccination strategies aimed at generating TRM cells have shown promise against a range of pathogens. We have previously shown that the choice of adjuvant critically influences CD8+ TRM cell formation in the liver. However, the range of adjuvants tested was limited. Here, we assessed the ability of a broad range of adjuvants stimulating membrane (TLR4), endosomal (TLR3, TLR7 and TLR9) and cytosolic (cGAS, RIG-I) pathogen recognition receptors for their capacity to induce CD8+ TRM formation in a subunit vaccination model. We show that CpG oligodeoxynucleotides (ODN) remain the most efficient inducers of liver TRM cells among all adjuvants tested. Moreover, their combination with the cationic liposome DOTAP further enhances the potency, particularly of the class B ODN CpG 1668 and the human TLR9 ligand CpG 2006 (CpG 7909). This study informs the design of efficient liver TRM-based vaccines for their potential translation.
| Item ID: | 77321 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1873-2518 |
| Copyright Information: | © 2022 Elsevier Ltd. All rights reserved. |
| Funders: | National Health and Medical Research Council of Australia (NHMRC) |
| Projects and Grants: | NHMRC 1139486, NHMRC 1154457, NHMRC 1113293, NHMRC 1124706 |
| Date Deposited: | 25 Jan 2023 05:23 |
| FoR Codes: | 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320404 Cellular immunology @ 100% |
| SEO Codes: | 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280103 Expanding knowledge in the biomedical and clinical sciences @ 100% |
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