Next Generation Sequencing of Genotype Variants and Genetic Association between Heat Shock Proteins HSPA1B Single Nucleotide Polymorphism at the g.31829044 Locus and Heat Tolerance: A Pilot Quasi-Experimental Study

Alele, Faith O., Otto, John R., Malau-Aduli, Bunmi S., and Malau-Aduli, Aduli E.O. (2022) Next Generation Sequencing of Genotype Variants and Genetic Association between Heat Shock Proteins HSPA1B Single Nucleotide Polymorphism at the g.31829044 Locus and Heat Tolerance: A Pilot Quasi-Experimental Study. Biomolecules, 12 (10). 1465.

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Abstract

Heat tolerance and exertional heat stroke (EHS) are rare health conditions that have been described and characterised but have never been genetically solved. Knowledge of the role of single nucleotide polymorphisms (SNPs) in heat shock proteins (HSPs) genes and their associations with heat tolerance and EHS is limited. This pilot study aimed to identify SNP in HSPA1B, HSP90AA2 and DNAJA1 genes and their associations with heat tolerance and EHS history in a quasi-experimental design. Participants comprised Australian Defence Force members (ADF) who had a history of EHS and the general population. Genomic DNA samples were extracted from the venous blood samples of 48 participants, sequenced and analysed for SNP. Forty-four per cent (44%) of the participants were heat intolerant, and 29% had a history of EHS. Among participants with a history of EHS, there was an association between heat tolerance and HSPA1B SNP at the g.31829044 locus. However, there were no associations between HSPA1B and HSP90AA2 SNP and heat tolerance. All participants had the same distribution for the DNAJA1 SNP. In conclusion, the findings indicate an association between the HSPA1B genetic variant at the g.31829044 locus and heat tolerance among ADF participants with a history of EHS. Further research with a larger number of military participants will shed more light on the associations between HSP genes and heat tolerance.

Item ID: 76291
Item Type: Article (Research - C1)
ISSN: 2218-273X
Keywords: heat tolerance; heat shock proteins; HSPA1B; HSP90AA2; exertional heat stroke; exertional heat illness; military; genetics; polymorphism
Copyright Information: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
Research Data: https://research.jcu.edu.au/data/published/abd6de00799b11ecae4bb7cd799d3a4a/
Date Deposited: 12 Oct 2022 23:45
FoR Codes: 42 HEALTH SCIENCES > 4206 Public health > 420603 Health promotion @ 50%
31 BIOLOGICAL SCIENCES > 3105 Genetics > 310509 Genomics @ 50%
SEO Codes: 14 DEFENCE > 1401 Defence > 140110 Personnel @ 50%
20 HEALTH > 2002 Evaluation of health and support services > 200201 Determinants of health @ 50%
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