Molecular identification of trematode parasites infecting the freshwater snail Bithynia siamensis goniomphalos in Thailand
Pitaksakulrat, O., Sithithaworn, P., Kopolrat, K.Y., Kiatsopit, N., Saijuntha, W., Andrews, R.H., Petney, T.N., and Blair, D. (2022) Molecular identification of trematode parasites infecting the freshwater snail Bithynia siamensis goniomphalos in Thailand. Journal of Helminthology, 96. e49.
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Abstract
Digenetic trematodes are important parasites of humans and animals. They have complex life cycles and typically infect a gastropod as the first intermediate host. Bithynia siamensis goniomphalos, the first intermediate host of the liver fluke, Opisthorchis viverrini, harbours a wide variety of other trematode species. Morphological details of cercariae of 20 trematode taxa from B. s. goniomphalos, collected mainly in Thailand from 2009 to 2014, were provided in an earlier paper. Correct identification to the species or genus level based on morphology of these cercariae is generally not possible. Therefore, we used molecular data to improve identification and to investigate the diversity of the species of trematodes infecting B. s. goniomphalos. We were successful in extracting, amplifying and sequencing portions of the 28S ribosomal RNA (rRNA) gene for 19 of these 20 types of cercaria, and the internal transcribed spacer 2 region for 18 types. BLAST searches in GenBank and phylogenetic trees inferred from the 28S rRNA sequences identified members of at least nine superfamilies and 12 families. Only a few cercariae could be assigned confidently to genus or species on the basis of the sequence data. Matching sequence data from named adult trematodes will be required for definitive identification. There is clearly a great diversity of trematode species utilizing B. s. goniomphalos in Thailand.
Item ID: | 75639 |
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Item Type: | Article (Research - C1) |
ISSN: | 1475-2697 |
Keywords: | 28s rDNA, Bithynia siamensis goniomphalos, cercariae, molecular identification, Opisthorchis viverrini |
Copyright Information: | Copyright © The Author(s), 2022. Published by Cambridge University Press |
Date Deposited: | 03 Aug 2022 09:58 |
FoR Codes: | 31 BIOLOGICAL SCIENCES > 3109 Zoology > 310913 Invertebrate biology @ 50% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3299 Other biomedical and clinical sciences > 329999 Other biomedical and clinical sciences not elsewhere classified @ 50% |
SEO Codes: | 20 HEALTH > 2004 Public health (excl. specific population health) > 200404 Disease distribution and transmission (incl. surveillance and response) @ 40% 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280103 Expanding knowledge in the biomedical and clinical sciences @ 60% |
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