The effects of feeding ration and cheliped autotomy on the growth and expression of ecdysteroid receptor in early juvenile mud crabs, Scylla paramamosain

Gong, Jie, Huang, Chencui, Yu, Kun, Li, Shaojing, Zeng, Chaoshu, and Ye, Haihui (2022) The effects of feeding ration and cheliped autotomy on the growth and expression of ecdysteroid receptor in early juvenile mud crabs, Scylla paramamosain. Aquaculture Reports, 25. 101206.

[img]
Preview
PDF (Published Version) - Published Version
Available under License Creative Commons Attribution.

Download (2MB) | Preview
View at Publisher Website: https://doi.org/10.1016/j.aqrep.2022.101...
 
13


Abstract

This study investigated the combined effects of feeding ration and cheliped autotomy on the intermolt duration, molting success, molt increments in size and weight, and ecdysteroid receptor gene (SpEcR) expression of early juvenile mud crab Scylla paramamosain. Newly molted second stage juvenile crabs (C2) were subjected to four feeding conditions; optimal, suboptimal (1/2 optimal), low (1/4 optimal) ration and starvation, and autotomy (intact vs. cheliped autotomy) in a 4 x 2 factorial design until all crabs successfully molted or died. A significant interaction of feeding ration and cheliped autotomy on intermolt duration was identified. With reduced feeding ration, both intact and cheliped autotomized crabs showed increased time and desynchrony of molting, but decreased carapace size and body weight. Importantly, all crabs with different feeding rations even the low ration had high rates of molting success (> 95%), while the crabs subjected to starvation died without molting. When fed optimal ration, the mean intermolt duration of the cheliped autotomized crabs was significantly prolonged, while no such effect was found between autotomized and intact crabs subjected to suboptimal or low feeding ration. The qRT-PCR revealed that the expression of SpEcR showed a general trend of inhibited by reduced feeding ration, which was consistent with observed significantly increased intermolt duration. Interestingly, the transcript level of SpEcR was only significantly affected by cheliped autotomy under the optimal and suboptimal feeding rations but not for the low feeding ration. Together, the results of this study suggest that the S. paramamosain early juveniles have a strong tolerance for fluctuations in food availability. In addition, the availability of food and limb autotomy could significantly affect growth, molting duration and synchrony of the crabs, which appeared to reflect in SpEcR expression level that involved in the regulation of molting and limb regeneration process of the juvenile crabs.

Item ID: 75608
Item Type: Article (Research - C1)
ISSN: 2352-5134
Keywords: Food availability, Autotomy, Growth, Tolerance, Scylla paramamosain
Copyright Information: © 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Date Deposited: 03 Aug 2022 07:36
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 100%
SEO Codes: 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1002 Fisheries - aquaculture > 100201 Aquaculture crustaceans (excl. rock lobster and prawns) @ 100%
Downloads: Total: 13
Last 12 Months: 4
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page