COPD is associated with elevated IFN-β production by bronchial epithelial cells infected with RSV or hMPV

Collinson, Natasha, Snape, Natale, Beagley, Kenneth, Fantino, Emmanuelle, and Spann, Kirsten (2021) COPD is associated with elevated IFN-β production by bronchial epithelial cells infected with RSV or hMPV. Viruses, 13 (5). 911.

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Abstract

IFN treatment may be a viable option for treating COPD exacerbations based on evidence of IFN deficiency in COPD. However, in vitro studies have used primarily influenza and rhinoviruses to investigate IFN responses. This study aims to investigate the susceptibility to infection and IFN response of primary bronchial epithelial cells (BECs) from COPD donors to infection with RSV and hMPV. BECs from five COPD and five healthy donors were used to establish both submerged monolayer and well-differentiated (WD) cultures. Two isolates of both RSV and hMPV were used to infect cells. COPD was not associated with elevated susceptibility to infection and there was no evidence of an intrinsic defect in IFN production in either cell model to either virus. Conversely, COPD was associated with significantly elevated IFN-β production in response to both viruses in both cell models. Only in WD-BECs infected with RSV was elevated IFN-β associated with reduced viral shedding. The role of elevated epithelial cell IFN-β production in the pathogenesis of COPD is not clear and warrants further investigation. Viruses vary in the responses that they induce in BECs, and so conclusions regarding antiviral responses associated with disease cannot be made based on single viral infections.

Item ID: 74890
Item Type: Article (Research - C1)
ISSN: 1999-4915
Keywords: Chronic obstructive pulmonary disease, Epithelial cells, Interferon, Metapneumovirus, Respiratory syncytial virus
Copyright Information: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Date Deposited: 05 Jul 2022 01:56
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3201 Cardiovascular medicine and haematology > 320103 Respiratory diseases @ 50%
32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320405 Humoural immunology and immunochemistry @ 20%
31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310706 Virology @ 30%
SEO Codes: 20 HEALTH > 2001 Clinical health > 200104 Prevention of human diseases and conditions @ 100%
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