Does Opisthorchis viverrini circulate between humans and domestic cats in an endemic area in Thailand?

Sota, Pornphutthachat, Suttiprapa, Sutas, Tangkawattana, Sirikachorn, Sripa, Manop, Blair, David, and Sripa, Banchob (2022) Does Opisthorchis viverrini circulate between humans and domestic cats in an endemic area in Thailand? Parasitology. (In Press)

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Abstract

The liver fluke Opisthorchis viverrini is a foodborne trematode that, in chronic infection, is a leading cause of bile-duct cancer cholangiocarcinoma (CCA). Cats and dogs are acknowledged as reservoir hosts of this parasite. However, this assumption is based on morphological similarity of flukes recovered from these hosts, without any molecular-genetic evidence. The aim of this study was to obtain molecular data from O. viverrini eggs present in feces of humans and cats in the same locality in Thanya Sub-district, Kalasin, Thailand. The mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was used as the marker for a population-genetic study. A DNA fragment of the cox1 gene was amplified from stool samples and subjected to nucleotide sequencing. Phylogenetic and haplotype network analyses were performed. The cox1 sequences of O. viverrini eggs from humans and cats largely formed separate clades on the phylogenetic trees, with an Fst value of 0.64 (p <0.05), indicating largely distinct populations in the two species. However, five samples from cats were placed in the human cluster and one sample from a human was placed in the cat cluster. This suggests that host specificity of "human" and "cat" clades is not absolute. These results indicate that there are two populations of O. viverrini, one circulates primarily in humans and the other in cats. However, cross-Transmission can occur between these two hosts. Taken altogether, the population-genetic evidence from this study partially supports the assumption that the cat can act as a reservoir host of O. viverrini.

Item ID: 74668
Item Type: Article (Research - C1)
ISSN: 1469-8161
Keywords: cat, human, Opisthorchis viverrini, population genetics, reservoir host
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Copyright Information: © The Author(s), 2022. Published by Cambridge University Press
Date Deposited: 14 Jun 2022 04:00
FoR Codes: 42 HEALTH SCIENCES > 4202 Epidemiology > 420207 Major global burdens of disease @ 25%
42 HEALTH SCIENCES > 4206 Public health > 420699 Public health not elsewhere classified @ 75%
SEO Codes: 20 HEALTH > 2004 Public health (excl. specific population health) > 200405 Food safety @ 50%
20 HEALTH > 2004 Public health (excl. specific population health) > 200404 Disease distribution and transmission (incl. surveillance and response) @ 50%
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