Molecular detection of six viral pathogens from Australian wild sourced giant black tiger shrimp (Penaeus monodon) broodstock

Arbon, P.M., Condon, K., Andrade Martinez, M., and Jerry, D.R. (2022) Molecular detection of six viral pathogens from Australian wild sourced giant black tiger shrimp (Penaeus monodon) broodstock. Aquaculture, 548. 737651.

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Abstract

Disease presents a considerable challenge to the sustainability and development of global shrimp aquaculture. The Australian black tiger shrimp (Penaeus monodon) aquaculture industry is heavily reliant on wild-sourced broodstock for seedstock production, representing a large and under evaluated biosecurity risk. Currently, there is a paucity of quantitative, large-scale data detailing the extent of pathogenic agents in wild-sourced P. monodon broodstock. This study presents a comprehensive investigation of the presence and level of detection of endemic pathogen targets in wild-sourced P. monodon broodstock collected from the two primary sources of supply for the Australian shrimp aquaculture industry using quantitative polymerase chain reaction (qPCR). Broodstock pleopod samples (N = 7472) were analysed by TaqMan qPCR for the detection of six viral pathogens. In total, 44.9% of wild-sourced broodstock were positive for the detection of at least one pathogen target. White spot syndrome virus and yellow head virus-1 were not detected in any sample. Infectious hypodermal haematopoietic necrosis virus (30%) and gill-associated virus (28.1%) were the most prevalent pathogen targets detected. Whenzhou (syn. Wenzhou) shrimp virus-2 and yellow head virus-7 were each cumulatively detected in less than 3% of broodstock samples. Geographic source, sex and year of collection of broodstock significantly influenced prevalence of detection. The current study will be valuable to the Australian shrimp aquaculture industry to improve understanding of the presence of pathogens in wild-sourced broodstock, along with informing management decisions related to wild broodstock collection and associated biosecurity practices.

Item ID: 74613
Item Type: Article (Research - C1)
ISSN: 1873-5622
Keywords: Broodstock, Penaeus monodon, Shrimp aquaculture, TaqMan qPCR, Viral pathogens
Copyright Information: © 2021 Elsevier B.V. All rights reserved.
Date Deposited: 10 Jun 2022 02:40
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 100%
SEO Codes: 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1002 Fisheries - aquaculture > 100205 Aquaculture prawns @ 100%
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