Baseline distribution of stable copper isotope compositions of the brain and other organs in mice

Moynier, Frédéric, Merland, Ariane, Rigoussen, Dimitri, Moureau, Julien, Paquet, Marine, Mahan, Brandon, and LeBorgne, Marie (2022) Baseline distribution of stable copper isotope compositions of the brain and other organs in mice. Metallomics, 14 (5). mfac017.

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Copper (Cu) stable isotopes are useful for understanding pathways and tracing changes in Cu homeostasis, such as those induced by various diseases (e.g. liver cirrhosis, numerous forms of cancer, and neurodegenerative diseases). However, this utility relies on a baseline understanding of the natural distribution of Cu isotopes between organs of healthy organisms, which is not well-known at present. Here, the distribution of natural Cu isotopes in the brain, liver, red blood cells, plasma, kidneys, and muscle of 14 mice (7 males and 7 females) from three different genetic backgrounds is assessed. We show that the Cu isotopic composition of most mouse organs is isotopically distinct from one another. The most striking feature is the heavy isotope enrichment of the kidney (δ65Cu = 1.65 ± 0.06‰, 2SE), brain (δ65Cu = 0.87 ± 0.03‰, 2SE) and liver (δ65Cu = 0.71 ± 0.24‰, 2SE) compared to blood components, i.e. red blood cells (RBCs) (δ65Cu = 0.30 ± 0.06‰, 2SE), and plasma (δ65Cu = –0.61 ± 0.08‰, 2SE), with δ65Cu being the per mil deviation of the 65Cu/63Cu ratio from the NIST SRM 976 standard. Differences in genetic background do not appear to affect the isotopic distribution of Cu. Interestingly, male and female mice appear to have different Cu concentrations and isotopic compositions in their brain, plasma, muscle, and RBC.

By demonstrating that organs have distinct isotopic compositions, our study reinforces the notion that Cu stable isotopes can be used to trace changes in homeostasis in diseases affecting Cu distribution, such as Alzheimer's disease, liver cancer, and possible chronic kidney failure.

Item ID: 73111
Item Type: Article (Research - C1)
ISSN: 1756-591X
Copyright Information: © The Author(s) 2022. Published by Oxford University Press. All rights reserved. This is a pre-copyedited, author-produced version of an article accepted for publication in Metallomics following peer review. The version of record Frédéric Moynier, Ariane Merland, Dimitri Rigoussen, Julien Moureau, Marine Paquet, Brandon Mahan, Marie Le Borgne, Baseline distribution of stable copper isotope compositions of the brain and other organs in mice, Metallomics, Volume 14, Issue 5, May 2022, mfac017 is available online at:
Date Deposited: 18 Jul 2022 06:29
FoR Codes: 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310101 Analytical biochemistry @ 50%
37 EARTH SCIENCES > 3703 Geochemistry > 370303 Isotope geochemistry @ 50%
SEO Codes: 20 HEALTH > 2001 Clinical health > 200101 Diagnosis of human diseases and conditions @ 50%
28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280103 Expanding knowledge in the biomedical and clinical sciences @ 50%
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