A potent vaccine delivery system
Zhao, Guangzu, Azuar, Armira, Toth, Istvan, and Skwarczynski, Mariusz (2021) A potent vaccine delivery system. Bio-protocol, 11 (7). e3973.
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Abstract
Most vaccines require co-delivery of an adjuvant in order to generate the desired immune responses. However, many currently available adjuvants are non-biodegradable, have limited efficacy, and/or poor safety profile. Thus, new adjuvants, or self-adjuvanting vaccine delivery systems, are required. Here, we proposed a self-adjuvanting delivery system that is fully defined, biodegradable, and non-toxic. The system is produced by conjugation of polyleucine to peptide antigen, followed by self-assembly of the conjugate into nanoparticles. The protocol includes solid-phase peptide synthesis of the vaccine conjugate, purification, self-assembly and physicochemical characterization of the product. Overall, this protocol describes, in detail, the production of a well-defined and effective self-adjuvanting delivery system for peptide antigens, along with tips for troubleshooting.
| Item ID: | 71246 |
|---|---|
| Item Type: | Article (Scholarly Work) |
| ISSN: | 2331-8325 |
| Keywords: | Poly (hydrophobic amino acid), Delivery system, Subunit vaccine, Peptide synthesis, Particle self-assembly, Self-adjuvanting nanoparticles, Polyleucine, Secondary structure |
| Date Deposited: | 26 May 2022 00:04 |
| FoR Codes: | 32 BIOMEDICAL AND CLINICAL SCIENCES > 3206 Medical biotechnology > 320604 Nanomedicine @ 100% |
| SEO Codes: | 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280103 Expanding knowledge in the biomedical and clinical sciences @ 100% |
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