Protein extraction protocols for optimal proteome measurement and arginine kinase quantitation from cricket Acheta domesticus for foodsafety assessment
Bose, Utpal, Broadbent, James A., Juhász, Angéla, Karnaneedi, Shaymaviswanathan, Johnston, Elecia B., Stockwell, Sally, Byrne, Keren, Limviphuvadh, Vachiranee, Maurer-Stroh, Sebastian, Lopata, Andreas L., and Colgrave, Michelle L. (2021) Protein extraction protocols for optimal proteome measurement and arginine kinase quantitation from cricket Acheta domesticus for foodsafety assessment. Food Chemistry, 348. 129110.
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Abstract
Insects have been consumed by people for millennia and have recently been proposed as a complementary, sustainable source of protein to feed the world’s growing population. Insects and crustaceans both belong to the arthropod family. Crustacean (shellfish) allergies are common and potentially severe; hence, the cross-reactivity of the immune system with insect proteins is a potential health concern. Herein, LC-MS/MS was used to explore the proteome of whole, roasted whole and roasted powdered cricket products. Eight protein extraction protocols were compared using the total number of protein and distinct peptide identifications. Within these data, 20 putative allergens were identified, of which three were arginine kinase (AK) proteoforms. Subsequently, a multiple reaction monitoring MS assay was developed for the AK proteoforms and applied to a subset of extracts. This targeted assay demonstrated that allergen abundance/detectability varies according to the extraction method as well as the food processing method.
| Item ID: | 70882 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1873-7072 |
| Keywords: | Cricket, Protein extraction, Protocol optimisation, Proteomics, LC-MRM-MS, Allergen, Cross-reactive, Arginine kinase |
| Copyright Information: | © 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license. |
| Date Deposited: | 07 Dec 2021 23:29 |
| FoR Codes: | 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320401 Allergy @ 50% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3205 Medical biochemistry and metabolomics > 320506 Medical biochemistry - proteins and peptides (incl. medical proteomics) @ 50% |
| SEO Codes: | 20 HEALTH > 2004 Public health (excl. specific population health) > 200405 Food safety @ 50% 20 HEALTH > 2001 Clinical health > 200199 Clinical health not elsewhere classified @ 50% |
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