Visualization of cyanogenic glycosides in floral tissues

Ritmejeryte, Edita, Miller, Rebecca E., Bayly, Michael J., and Boughton, Berin A. (2022) Visualization of cyanogenic glycosides in floral tissues. In: Beale, David J., Hillyer, Katie, Warden, Andrew, and Jones, Oliver, (eds.) Applied Environmental Metabolomics - Community Insights and Guidance from the Field. Elsevier, London, United Kingdom, pp. 29-44.

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Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a particularly useful tool to determine the localization of metabolites in plant tissues enabling the exploration of spatial metabolic processes in plant biology. In this case study, we examined cyanogenic glycosides (CNglycs), in the delicate floral tissues of highly cyanogenic Grevillea robusta (southern silky oak) and Macadamia tetraphylla (macadamia nut) collected in the field. CNglycs are canonical defence metabolites, but more recent work indicates they may play a much broader role in plant metabolism. We quantified CNglycs in floral tissues by chemical assay and visualized their spatial distribution using MALDI-MSI to explore their biochemical and ecological roles. The species examined contained two tyrosine-derived CNglycs, the monoglycoside dhurrin, and diglycoside proteacin, identified using HP-TLC and LC-MS/MS analyses. Within florets, the most cyanogenic tissue was the style for both species. MALDI-MSI revealed different distributions of dhurrin and proteacin within florets of both species and differences in the ratios of dhurrin to proteacin between specific floral tissues. Dhurrin tended to be mostly localized in epidermal layers, consistent with a role in defence, while proteacin was distributed more evenly throughout other floral tissues indicating other potential roles.

Item ID: 69599
Item Type: Book Chapter (Research - B1)
ISBN: 978-0-12-816460-0
Copyright Information: Copyright © 2022 Elsevier Inc. All rights reserved.
Sensitivity Note: The work may only be shared by the publisher.
Date Deposited: 01 Sep 2022 00:59
FoR Codes: 34 CHEMICAL SCIENCES > 3401 Analytical chemistry > 340105 Instrumental methods (excl. immunological and bioassay methods) @ 50%
34 CHEMICAL SCIENCES > 3401 Analytical chemistry > 340199 Analytical chemistry not elsewhere classified @ 20%
31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310802 Plant biochemistry @ 30%
SEO Codes: 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280105 Expanding knowledge in the chemical sciences @ 50%
28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences @ 50%
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