Next generation sequencing of single nucleotide polymorphic DNA-markers in selecting for intramuscular fat, fat melting point, omega-3 long-chain polyunsaturated fatty acids and meat eating quality in Tattykeel Australian White MARGRA lamb

Pewan, Shedrach Benjamin, Otto, John Roger, Huerlimann, Roger, Budd, Alyssa Marie, Mwangi, Felista Waithira, Edmunds, Richard Crawford, Holman, Benjamin William Behrens, Henry, Michelle Lauren Elizabeth, Kinobe, Robert Tumwesigye, Adegboye, Oyelola Abdulwasiu, and Malau-Aduli, Aduli Enoch Othniel (2021) Next generation sequencing of single nucleotide polymorphic DNA-markers in selecting for intramuscular fat, fat melting point, omega-3 long-chain polyunsaturated fatty acids and meat eating quality in Tattykeel Australian White MARGRA lamb. Foods, 10 (10). 2288.

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Abstract

Meat quality data can only be obtained after slaughter when selection decisions about the live animal are already too late. Carcass estimated breeding values present major precision problems due to low accuracy, and by the time an informed decision on the genetic merit for meat quality is made, the animal is already dead. We report for the first time, a targeted next-generation sequencing (NGS) of single nucleotide polymorphisms (SNP) of lipid metabolism genes in Tattykeel Australian White (TAW) sheep of the MARGRA lamb brand, utilizing an innovative and minimally invasive muscle biopsy sampling technique for directly quantifying the genetic worth of live lambs for health-beneficial omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA), intramuscular fat (IMF), and fat melting point (FMP). NGS of stearoyl-CoA desaturase (SCD), fatty acid binding protein-4 (FABP4), and fatty acid synthase (FASN) genes identified functional SNP with unique DNA marker signatures for TAW genetics. The SCD g.23881050T>C locus was significantly associated with IMF, C22:6n-3, and C22:5n-3; FASN g.12323864A>G locus with FMP, C18:3n-3, C18:1n-9, C18:0, C16:0, MUFA, and FABP4 g.62829478A>T locus with IMF. These add new knowledge, precision, and reliability in directly making early and informed decisions on live sheep selection and breeding for health-beneficial n-3 LC-PUFA, FMP, IMF and superior meat-eating quality at the farmgate level. The findings provide evidence that significant associations exist between SNP of lipid metabolism genes and n-3 LC-PUFA, IMF, and FMP, thus underpinning potential marker-assisted selection for meat-eating quality traits in TAW lambs.

Item ID: 69456
Item Type: Article (Research - C1)
ISSN: 2304-8158
Keywords: SNP; FASN; SCD; FABP4; IMF; FMP; eating quality; TAW MARGRA lamb; biopsy; n‐3 LC‐PUFA
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Copyright Information: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Date Deposited: 28 Sep 2021 02:08
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3003 Animal production > 300305 Animal reproduction and breeding @ 50%
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3003 Animal production > 300399 Animal production not elsewhere classified @ 50%
SEO Codes: 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1004 Livestock raising > 100412 Sheep for meat @ 100%
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