2-DE profiling of GDNF overexpression-related proteome changes in differentiating ST14A rat progenitor cells

Hoffrogge, Raimund, Beyer, Susanne, Hübner, Rayk, Mikkat, Stefan, Mix, Eilhard, Scharf, Christian, Schmitz, Ulf, Pauleweit, Stefan, Berth, Matthias, Zubrzycki, Igor Z., Christoph, Hilmar, Pahnke, Jens, Wolkenhauer, Olaf, Uhrmacher, Adelinde, Völker, Uwe, and Rolfs, Arndt (2007) 2-DE profiling of GDNF overexpression-related proteome changes in differentiating ST14A rat progenitor cells. Proteomics, 7 (1). pp. 33-46.

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Targeted differentiation of neural progenitor cells (NPCs) is a challenge for treatment of neurodegenerative diseases by cell replacement therapy and cell signalling manipulation. Here, we applied a proteome profiling approach to the rat striatal progenitor model cell line ST14A in order to elucidate cellular differentiation processes. Native cells and cells transfected with the glial cell line-derived neurotrophic factor (GDNF) gene were investigated at the proliferative state and at seven time points up to 72 h after induction of differentiation. 2-DE combined with MALDI-MS was used to create a reference 2-DE-map of 652 spots of which 164 were identified and assigned to 155 unique proteins. For identification of protein expression changes during cell differentiation, spot patterns of triplicate gels were matched to the 2-DE-map. Besides proteins that display expression changes in native cells, we also noted 43 protein-spots that were differentially regulated by GDNF overexpression in more than four time points of the experiment. The expression patterns of putative differentiation markers such as annexin 5 (ANXA5), glucosidase II beta subunit (GLU2B), phosphatidylethanolamine-binding protein 1 (PEBP1), myosin regulatory light chain 2-A (MLRA), NASCENT polypeptide-associated complex alpha (NACA), elongation factor 2 (EF2), peroxiredoxin-1 (PRDX1) and proliferating cell nuclear antigen (PCNA) were verified by Western blotting. The results reflect the large rearrangements of the proteome during the differentiation process of NPCs and their strong modification by neurotrophic factors like GDNF.

Item ID: 69029
Item Type: Article (Research - C1)
ISSN: 1615-9861
Copyright Information: Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Date Deposited: 21 Mar 2024 04:50
FoR Codes: 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310109 Proteomics and intermolecular interactions (excl. medical proteomics) @ 100%
SEO Codes: 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280102 Expanding knowledge in the biological sciences @ 100%
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