Alternative feed raw materials modulate intestinal microbiota and its relationship with digestibility in Yellowtail Kingfish Seriola lalandi

Dam, Chinh Thi My, Booth, Mark, Pirozzi, Igor, Salini, Michael, Smullen, Richard, Ventura, Tomer, and Elizur, Abigail (2020) Alternative feed raw materials modulate intestinal microbiota and its relationship with digestibility in Yellowtail Kingfish Seriola lalandi. Fishes, 5 (2). 14.

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Abstract

Gut microbiota plays a crucial role in nutrient digestibility and fish health. This study aimed to investigate the effects of alternative feed raw materials on the bacterial communities in the distal intestine and its relationship with nutrient digestibility in yellowtail kingfish (YTK), Seriola lalandi. Two 4-week digestibility trials were conducted to evaluate fish meal (FM), two sources of poultry by-product meal (PBM-1 & PBM-2), blood meal (BLM), faba bean meal (FBM), corn gluten meal (CGM), soy protein concentrate (SPC) and wheat flour (WH). The nutrient digestibility value was determined using the stripping fecal collection method. Bacterial communities were characterized by high-throughput sequencing based on V3-V4 region of the 16S rRNA gene. The most abundant phylum identified in the present study was Proteobacteria. A significant change in the distal intestine was observed in fish fed diets containing CGM and BLM, characterized by a reduction of species richness and diversity. Additionally, significant correlation between nutrient digestibility and intestinal microbiota was observed. Allivibrio, Vibrio, Curvibacter, Ruminococcaceae, and Clostridium were positively correlated, whereas Ralstonia genus was negatively correlated with nutrient digestibility. This study demonstrated that intestinal microbiota could be a useful tool for evaluating the digestibility of feed raw materials; however, further culture-based study is needed to confirm this observation.

Item ID: 67183
Item Type: Article (Research - C1)
ISSN: 2410-3888
Keywords: 16S rRNA gene amplicon sequencing, Alternative raw feed materials, Digestibility, Distal intestinal microbiota, Fish nutrition, Yellowtail kingfish
Copyright Information: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Funders: Department of Agriculture and Water Resources (DAWR), Fisheries Research and Development Corporation (FRDC), Huon Aquaculture, Clean Seas Seafood
Projects and Grants: DAWR RnD4Profit-14-01-027
Date Deposited: 08 Apr 2021 23:18
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 100%
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