Intestinal transcriptome analysis highlights key differentially expressed genes involved in nutrient metabolism and digestion in yellowtail kingfish (Seriola lalandi) fed terrestrial animal and plant proteins

Dam, Chinh Thi My, Ventura, Tomer, Booth, Mark, Pirozzi, Igor, Salini, Michael, Smullen, Richard, and Elizur, Abigail (2020) Intestinal transcriptome analysis highlights key differentially expressed genes involved in nutrient metabolism and digestion in yellowtail kingfish (Seriola lalandi) fed terrestrial animal and plant proteins. Genes, 11 (6). 621.

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Abstract

This study investigated the effects of dietary terrestrial animal and plant proteins on the intestinal transcriptomes of yellowtail kingfish (YTK), Seriola lalandi, an ecologically and economically important marine species in Australia. Five diets containing fish meal (FM), poultry by-product meal (PBM), blood meal (BLM), faba bean meal (FBM) and corn gluten meal (CGM) were formulated and fed over a period of 4 weeks. The Illumina RNA-sequencing (RNA-Seq) results identified a suite of differentially expressed genes involved in nutrient metabolism and protein digestion pathways, reinforced by quantitative polymerase chain reaction (qPCR) results. These findings provide molecular support to the notion that PBM and FBM are useful raw materials in commercial diets for YTK. Using the same evidence, we have demonstrated that BLM and CGM may be less useful and their incorporation into commercial aquafeeds for this species should be done cautiously. The differentially expressed genes showed a subtle difference and high correlation with apparent nutrient digestibility of raw materials. Further, our results indicate that transcriptome profiling provides a useful tool to evaluate alternative protein sources for use in aquaculture feeds.

Item ID: 67182
Item Type: Article (Research - C1)
ISSN: 2073-4425
Keywords: Alternative protein sources, Digestion, Distal intestine, RNA-sequencing, Transcriptomes, Yellowtail kingfish
Copyright Information: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Funders: Australian Government Department of Agriculture and Water Resources (AG), Fisheries Research and Development Corporation (FRDC), South Australian Research and Development Institute (SARDI), Clean Seas Seafood, NSW Department of Primary Industries (NSW DPI), Huon Aquaculture, Vietnam International Education Development (VIED)/University of the Sunshine Coast (USC)
Projects and Grants: AG Rural R&D for Profit program, FRDC Project No. 2016-200.30, NSW DPI Project No. 2016-200.20, VIED/USC PhD stipend
Date Deposited: 12 May 2021 23:35
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3005 Fisheries sciences > 300501 Aquaculture @ 100%
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