Metabolomes and lipidomes of the infective stages of the gastrointestinal nematodes, Nippostrongylus brasiliensis and Trichuris muris

Yeshi, Karma, Creek, Darren J., Anderson, Dovile, Ritmejerytė, Edita, Becker, Luke, Loukas, Alex, and Wangchuk, Phurpa (2020) Metabolomes and lipidomes of the infective stages of the gastrointestinal nematodes, Nippostrongylus brasiliensis and Trichuris muris. Metabolites, 10 (11). 446.

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Abstract

Soil-transmitted helminths, including hookworms and whipworms, infect billions of people worldwide. Their capacity to penetrate and migrate through their hosts’ tissues is influenced by the suite of molecules produced by the infective developmental stages. To facilitate a better understanding of the immunobiology and pathogenicity of human hookworms and whipworms, we investigated the metabolomes of the infective stage of Nippostrongylus brasiliensis third-stage larvae (L3) which penetrate the skin and Trichuris muris eggs which are orally ingested, using untargeted liquid chromatography–mass spectrometry (LC-MS). We identified 55 polar metabolites through Metabolomics Standard Initiative level-1 (MSI-I) identification from N. brasiliensis and T. muris infective stages, out of which seven were unique to excretory/secretory products (ESPs) of N. brasiliensis L3. Amino acids were a principal constituent (33 amino acids). Additionally, we identified 350 putative lipids, out of which 28 (all known lipids) were unique to N. brasiliensis L3 somatic extract and four to T. muris embryonated egg somatic extract. Glycerophospholipids and glycerolipids were the major lipid groups. The catalogue of metabolites identified in this study shed light on the biology, and possible therapeutic and diagnostic targets for the treatment of these critical infectious pathogens. Moreover, with the growing body of literature on the therapeutic utility of helminth ESPs for treating inflammatory diseases, a role for metabolites is likely but has received little attention thus far.

Item ID: 64997
Item Type: Article (Research - C1)
ISSN: 2218-1989
Keywords: Helminths; infective L3 stage; excretory-secretory products; lipidomes and metabolomes; Pharmacological properties
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Copyright Information: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Funders: James Cook University (JCU), National Health and Medical Research Council (NHMRC), Australian Institute of Tropical Health and Medicine (AITHM)
Projects and Grants: JCU Postgraduate Research Scholarship, NHMRC Ideas Grant (APP1183323), AITHM Capacity Development Grant, NHMRC Program Grant (APP1132975), NHMRC Senior Principal Research Fellowship (APP1117504)
Date Deposited: 09 Nov 2020 22:46
FoR Codes: 34 CHEMICAL SCIENCES > 3404 Medicinal and biomolecular chemistry > 340401 Biologically active molecules @ 50%
46 INFORMATION AND COMPUTING SCIENCES > 4606 Distributed computing and systems software > 460606 Energy-efficient computing @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 50%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 50%
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