A ratiometry-induced successive reusable electrochemical aptasensing platform: efficient monitoring of aflatoxin B1 in peanut

Li, Yuye, Liu, Dong, Zhu, Chengxi, Wang, Meng, Liu, Yang, and You, Tianyan (2021) A ratiometry-induced successive reusable electrochemical aptasensing platform: efficient monitoring of aflatoxin B1 in peanut. Sensors and Actuators B: Chemical, 336. 129021.

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Currently, most reusable aptasensors rely on the reassembly of aptamers to regenerate the sensing interface. However, it remains challenging to achieve successive aptasensor reusability. Herein, for the first time, we demonstrate that a ratiometric strategy endows the electrochemical aptasensor high reusability to realize the successive detection of aflatoxin B1 (AFB1). The model sensing interface used thionine (THI)-graphene nanocomposite and ferrocene (Fc)-labeled aptamer to output current signals (ITHI and IFc). For analysis, the specific recognition of AFB1 by aptamers caused it to strip from the electrode, and the value of ITHI/IFc varied linearly with AFB1 concentration over the 0.01-100 ng mL-1 range. This relationship also worked when using a single sensor for multiple successive testing but completely failed with ITHI or IFc alone. This unique successive reusability was ascribed to the ratiometric strategy capable of eliminating the environmental influence on both signals. The feasibility of the proposed aptasensor was validated by applying it to the successive detection of AFB1, which showed excellent accuracy and reliability compared with the official method, i.e., standard high-performance liquid chromatography-fluorescence (HPLC-FL). This successive reusable electrochemical aptasensing platform can be universal for advanced analysis, and it promotes the practical applications of aptasensors particularly in the field of rapid assays.

Item ID: 64996
Item Type: Article (Research - C1)
ISSN: 1873-3077
Keywords: Ratiometry; Electrochemical aptasensor; Successive reusability; Aflatoxin B1
Copyright Information: Accepted Version: © 2020 Published by Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Funders: National Natural Science Foundation of China (NNSFC), Priority Academic Program Development of Jiangsu Higher Education Institutions, the Innovation/Entrepreneurship Program of Jiangsu Province, Six Categories Talent Peak of Jiangsu Province (SCTPJP), Postgraduate Research & Practice Innovation Program of Jiangsu Province (PRPIPJP), Natural Science Foundation of Jiangsu Province of China (NSFJPC), Zhenjiang Key Laboratory of Advanced Sensing Materials and Devices (ZKLASMD)
Projects and Grants: NNSFC No. 61901193, 22074055, SCTPJP No. NY-011, PRPIPJP (KYCX20_3087), NSFJPC No. BK20160490, ZKLASMD No. SS2018001
Date Deposited: 24 Nov 2020 01:50
FoR Codes: 34 CHEMICAL SCIENCES > 3401 Analytical chemistry > 340103 Electroanalytical chemistry @ 50%
40 ENGINEERING > 4018 Nanotechnology > 401807 Nanomaterials @ 20%
34 CHEMICAL SCIENCES > 3406 Physical chemistry > 340604 Electrochemistry @ 30%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 80%
97 EXPANDING KNOWLEDGE > 970110 Expanding Knowledge in Technology @ 20%
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