Immobilisation of living coral embryos and larvae
Randall, Carly J., Giuliano, Christine, Mead, David, Heyward, Andrew J., and Negri, Andrew P. (2019) Immobilisation of living coral embryos and larvae. Scientific Reports, 9. 14596.
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Abstract
Embedding and immobilisation of living cells and microorganisms is used in a variety of research and commercial applications. Here we report the successful extended immobilisation of coral larvae in a low-gelling temperature agarose. Embryos and larvae of five broadcast-spawning Scleractinian species were immobilised in agarose gel and tested in a series of exploratory survival and settlement assays. The optimal developmental stage for immobilisation was after ciliation at approximately 24 hours post-fertilisation, after which, survival of immobilised larvae of all species was nearly 100%. In long-term assays, 50% of Montipora digitata larvae survived immobilised for 89 days. Furthermore, immobilised larvae of multiple species, that were released from the agarose, generally remained capable of settlement. These results demonstrate that the immobilisation of the early life-history stages of corals is possible for a variety of applications in basic and applied science.
| Item ID: | 64853 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 2045-2322 |
| Copyright Information: | © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
| Funders: | Australian Insitute of Marine Science (AIMS) |
| Projects and Grants: | AIMS Reef Restoration and Adaptation Program |
| Date Deposited: | 10 Jun 2021 01:17 |
| FoR Codes: | 31 BIOLOGICAL SCIENCES > 3109 Zoology > 310913 Invertebrate biology @ 100% |
| SEO Codes: | 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100% |
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