Quantitative proteomic analysis of the slime and ventral mantle glands of the Striped Pyjama squid (Sepioloidea lineolata)

Caruana, Nikeisha J., Strugnell, Jan M., Finn, Julian, Faou, Pierre, Plummer, Kim M., and Cooke, Ira R. (2020) Quantitative proteomic analysis of the slime and ventral mantle glands of the Striped Pyjama squid (Sepioloidea lineolata). Journal of Proteome Research, 19. pp. 1491-1501.

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Abstract

Cephalopods are known to produce an extensive range of secretions including ink, mucus, and venom. Sepiadariidae, a family of small, benthic bobtail squids, are notable for the high volume of viscous slime they emit when stressed. One species, Sepioloidea lineolata (striped pyjama squid), is covered with glands along the perimeter of the ventral mantle, and these structures are hypothesized to be the source of its slime. Using label-free quantitative proteomics, we analyzed five tissue types (dorsal and ventral mantle muscle, dorsal and ventral epithelium, and ventral mantle glands) and the slime from four individuals. In doing so, we were able to determine the relationship between the slime and the tissues as well as highlight proteins that were specifically identified within the slime and ventral mantle glands. A total of 28 proteins were identified to be highly enriched in slime, and these were composed of peptidases and protease inhibitors. Seven of these proteins contained predicted signal peptides, indicating classical secretion, with four proteins having no identifiable domains or similarity to any known proteins. The ventral mantle glands also appear to be the tissue with the closest overall proteomic composition to the slime; therefore, it is likely that the slime originates, at least in part, from these glands.

Item ID: 64747
Item Type: Article (Research - C1)
ISSN: 1535-3907
Keywords: Cephalopoda squid; enzymes; proteomics; quantitative; secretion; differential expression
Copyright Information: Copyright © 2020 American Chemical Society
Date Deposited: 22 Oct 2020 06:45
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) @ 100%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%
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