Channel catfish ovarian fluid differentially enhances blue catfish sperm performance

Myers, J.N., Bradford, A.J., Hallas, V.S., Lawson, L.L., Pitcher, T.E., Dunham, R.A., Butts, I.A.E., and Myers, Jaelen (2020) Channel catfish ovarian fluid differentially enhances blue catfish sperm performance. Theriogenology, 149. pp. 62-71.

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Abstract

For externally fertilizing fishes, interactions between male and female gametes have been shown to have remarkable impacts on sperm performance. Ovarian fluid (OF) and its ability to alter the swimming behavior of fish sperm makes it a determining factor of fertility. With the expansion of channel catfish (Ictalurus punctatus) ♀ × blue catfish (Ictalurus furcatus) ♂ hybrid aquaculture, it is essential to understand the impacts during fertilization and the magnitude such gametic interactions have on sperm performance and subsequent male fertility potential. This study was conducted to address the following: 1) activate blue catfish sperm with/without channel catfish OF to determine impacts on sperm performance and 2) assess if sperm behave differently when activated in the OF from individual females. Sperm (n = 4 males) were activated without OF (control) and with diluted OF from unique females (n = 6), creating 24 experimental crosses. Sperm motility (%), velocity (VCL), and longevity were analyzed using computer assisted sperm analyses software. With OF incorporated in the activation media, sperm velocity was significantly higher than the control at 10, 20, and 30 s post-activation. OF did not have an impact on motility for any females at 10 s and 20 s post-activation but became significantly higher than the control at 30 s. In all cases, OF treatments greatly increased longevity. Male × female interactions were highly significant, such that motility, velocity, and longevity were dependent on specific male-female pairs. This information shows that OF should be incorporated in aquatic media to simulate natural spawning conditions and accurately assess the fluid mechanics of sperm propulsion for each male. Additionally, there are mechanisms that drive gamete interactions that need to be explored further, which may improve selection of male-female pairs for in-vitro fertilization. On a broad scale, our results also help to shed light on the complexities of fertilization and fish reproduction overall, which may have implications for recruitment variability and recovery strategies of threatened and/or endangered freshwater species.

Item ID: 64598
Item Type: Article (Research - C1)
ISSN: 1879-3231
Keywords: gamete interactions; hybrid catfish aquaculture; sperm motility; sperm behavior; artificial fertilization
Copyright Information: © 2020 Elsevier Inc. All rights reserved.
Funders: USDA National Institute of Food and Agriculture
Projects and Grants: Hatch project 1013854 (IAEB)
Date Deposited: 10 Nov 2020 21:02
FoR Codes: 31 BIOLOGICAL SCIENCES > 3109 Zoology > 310903 Animal developmental and reproductive biology @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830102 Aquaculture Fin Fish (excl. Tuna) @ 100%
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