Analysis of specific mRNA gene expression profiles as markers of egg and embryo quality for hybrid catfish aquaculture

Myers, Jaelen N., Dyce, Paul W., Chatakondi, Nagaraj G., Gorman, Sara A., Quiniou, Sylvie M.A., Su, Baofeng, Peatman, Eric, Dunham, Rex A., and Butts, Ian A.E. (2020) Analysis of specific mRNA gene expression profiles as markers of egg and embryo quality for hybrid catfish aquaculture. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 243. 110675.

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Abstract

Despite best efforts to optimize reproduction, egg incubation, and larval performance in captivity, inconsistencies in hatchery fish production are still created by high variations in egg quality from individual females. In some hatchery species, egg quality and generation of viable embryos are correlated to abundances of specific mRNAs. Channel catfish females show considerable extremes in egg quality, causing inconsistencies in channel catfish, Ictalurus punctatus, female × blue catfish, Ictalurus furcatus, male hybrid fry production. The objectives of this study were to examine relative transcripts linked to egg and embryo quality and determine expression between low-hatch and high-hatch egg batches through early development (0, 24, 48, and 96 h post-fertilization; HPF). RNA was extracted from eggs/embryos of nine females (n = 4 high-quality, n = 5 low-quality) and Real-Time PCR was used to quantify relative gene expression. The transcripts assessed in this study perform critical cellular functions, including tubulin β (tubb), cathepsin D (ctsd), cathepsin Z (ctsz), cathepsin B (ctsb), cyclin B (ccnb1), exportin-1 (xpo1), ring finger protein 213 (rnf213), glucocorticoid receptor-1 (GR-1), and heat shock protein 70 (hsp70). Relative gene expression of all transcripts except GR-1 and hsp70 were up-regulated in the high-hatch group and peaked at 48 HPF (neurulation stage), indicating the importance of these gene products at this threshold to normally progress until hatch. Due to lack of expression during earlier stages, maternally derived mRNAs for these genes do not seem to impact early embryonic development. Using mRNA markers as a selection mechanism for hatchery broodstock may lead to more high-hatch egg batches by reducing problems associated with poor egg quality.

Item ID: 64596
Item Type: Article (Research - C1)
ISSN: 1531-4332
Keywords: Hatch success; Embryo viability; Gene expression; Egg biomarker; Hybrid catfish; mRNA
Copyright Information: © 2020 Elsevier Inc. All rights reserved.
Funders: USDA National Institute of Food and Agriculture (NIFA), Alabama Agricultural Experiment Station (AAES)
Projects and Grants: NIFA Hatch project 1013854 (IAEB), AAES Young Investigator Research Program (IAEB)
Date Deposited: 28 Oct 2020 03:55
FoR Codes: 31 BIOLOGICAL SCIENCES > 3105 Genetics > 310505 Gene expression (incl. microarray and other genome-wide approaches) @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830102 Aquaculture Fin Fish (excl. Tuna) @ 100%
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