Isolation and characterization of a termite transferrin gene up-regulated on infection

Thompson, Graham J., Crozier, Y. Ching, and Crozier, Ross H. (2003) Isolation and characterization of a termite transferrin gene up-regulated on infection. Insect Molecular Biology, 12 (1). pp. 1-7.

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Abstract

PCR-based subtractive hybridization was used to isolate genes preferentially expressed in a termite (Mastotermes darwiniensis) following exposure to an entomopathogenic fungus. The subtraction procedure yielded a cDNA clone encoding a putative transferrin that, when sequenced to its ends, is the largest (728 amino acids) for any insect transferrin characterized to date. Cysteines and residues comprising putative iron-binding sites are conserved in both N- and C-terminal lobes, suggesting structural and functional similarity to diferric vertebrate transferrins. A quantitative PCR assay confirmed a significant increase in transferrin expression following infection, suggesting its up-regulation is part of the innate immune response. However, codon-based tests for selection among known insect transferrins revealed only a small proportion of codon-sites positively selected. Thus, unlike certain vertebrate transferrin lineages, no widespread evidence for pathogen-mediated positive selection was detected at this locus.

Item ID: 6414
Item Type: Article (Research - C1)
ISSN: 0962-1075
Keywords: insect immunity; Mastotermes; subtractive hybridization; tests for positive selection
Date Deposited: 25 Jan 2010 05:01
FoR Codes: 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060899 Zoology not elsewhere classified @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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