Intravenous labeling and analysis of the content of thymic perivascular spaces

Ruscher, Roland, and Hogquist, Kristin A. (2018) Intravenous labeling and analysis of the content of thymic perivascular spaces. Bio-protocol, 8 (5).

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View at Publisher Website: https://doi.org/10.21769/bioprotoc.2757
 
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Abstract

Following development in the thymus, T cells are thought to exit into the periphery predominantly through perivascular spaces (PVS). This exit route is used by conventional T cells, and likely also applies to unconventional T cell subsets, such as precursors of CD8αα and TCRγδ intraepithelial lymphocytes, regulatory T cells and natural killer T cells. Additional cell types might also be found in the PVS and initiate interactions with exiting T cells. The exact content of the PVS, and the processes within, are not well studied. To distinguish vascular from resident cells within various tissues by flow cytometry, intravenous (i.v.) labeling is becoming a commonly employed method. We recently used anti-CD45.2 antibodies and magnetic enrichment to further evaluate this technique, and compared labeled and unlabeled cells in the thymus and blood. This assay can be used to specifically investigate hematopoietic cell subsets within the PVS of the thymus.

Item ID: 63830
Item Type: Article (Scholarly Work)
ISSN: 2331-8325
Keywords: Perivascular spaces, Thymus, Thymic emigration, Recent thymic emigrants, Intravenous labeling
Copyright Information: © 2018 The Authors; exclusive licensee Bio-protocol LLC.
Funders: National Institute of Health (NIH), USA
Projects and Grants: NIH grant R37 AI39560, NIH grant PO1 AI35296
Date Deposited: 25 Aug 2021 00:23
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320499 Immunology not elsewhere classified @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 50%
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