The molecular weight of ulvan affects the in vitro inflammatory response of a murine macrophage

Kidgell, Joel T., Glasson, Christopher R.K., Magnusson, Marie, Vamvounis, George, Sims, Ian M., Carnachan, Susan M., Hinkley, Simon F.R., Lopata, Andreas L., de Nys, Rocky, and Taki, Aya C. (2020) The molecular weight of ulvan affects the in vitro inflammatory response of a murine macrophage. International Journal of Biological Macromolecules, 150. pp. 839-848.

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Abstract

Ulvan, a sulfated polysaccharide extracted from the green seaweed genus Ulva, has bioactive properties including an immunomodulating capacity. The immunomodulatory capacity of ulvan from Ulva ohnoi, however, has not been assessed in detail. We depolymerised purified ulvan from U. ohnoi to obtain a range of molecular weight fractions (Mw 7, 9, 13, 21, 209 kDa), which were characterised by constituent sugar analysis, SEC-MALLS, and NMR. Ulvan fractions contained 48.8–54.7 mol% rhamnose, 32.5–35.9 mol% glucuronic acid, 4.5–7.3 mol% iduronic acid, and 3.3–5.6mol% xylose. ¹Hand ¹³CNMR was consistent with hydrolysis occurring at the anomeric centre without further modification to the oligosaccharide structure. The in vitro immunomodulatory effect of ulvan fractions was quantified by measuring levels of inflammatory-mediating signalling molecules released from LPS-stimulated RAW264.7 murine macrophages. All ulvan fractions showed no toxicity on RAW264.7 cells at concentrations below 100 μgmL⁻¹ over 48 h. Secreted interleukin-10 and prostaglandin E2 demonstrated an anti-inflammatory effect by higher molecular weight ulvan fractions at 100 μgmL⁻¹. To a lesser extent, these fractions also enhanced the LPS-induced inflammation throughminor increases of IL-1β and IL-6. This study confirms that ulvan from U. ohnoi has a mild in vitro immunomodulatory effect.

Item ID: 63555
Item Type: Article (Research - C1)
ISSN: 1879-0003
Keywords: immunomodulation, molecular weight, sulfated polysaccharide
Copyright Information: © 2020 Elsevier B.V. All rights reserved.
Funders: James Cook University (JCU), Pacific Biotechnologies (PB), Australian Government (AG)
Projects and Grants: JCU PhD scholarship, PB Research and Development program for the Integrated Production of Macroalgae, AG Innovation Connections grant no. ICG000136
Date Deposited: 24 Jun 2020 22:02
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified @ 50%
03 CHEMICAL SCIENCES > 0303 Macromolecular and Materials Chemistry > 030399 Macromolecular and Materials Chemistry not elsewhere classified @ 50%
SEO Codes: 92 HEALTH > 9299 Other Health > 929999 Health not elsewhere classified @ 100%
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