Aptameric biosensor for the sensitive detection of major shrimp allergen, tropomyosin

Chinnappan, Raja, Rahamn, Anas Abdel, AlZabn, Razan, Kamath, Sandip, Lopata, Andreas L., Abu-Salah, Khalid M., and Zourob, Mohammed (2020) Aptameric biosensor for the sensitive detection of major shrimp allergen, tropomyosin. Food Chemistry, 314. 126133.

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Abstract

The development of a sensitive and rapid detection approach for allergens in various food matrices is essential to assist patients in managing their allergies. The most common methods used for allergen detection are based on immunoassays, PCR and mass spectrometry. However, all of them are very complex and time-consuming. Herein, an aptamer biosensor for the detection of the major shrimp allergen tropomyosin (TM) was developed. Graphene oxide (GO) was used as a platform for screening of the minimal-length aptamer sequence required for high-affinity target binding. A fluorescein dye labeled GO quenches the truncated aptamer by π-stacking interactions. After the addition of TM, the fluorescence was restored due to the competitive binding of the aptamer to GO. One of the truncated aptamers was found to bind to TM with four-fold higher affinity (30 nM) compared to the full-length aptamer (124 nM), with a limit of detection (LOD) of 2 nM. The aptamer-based sensor demonstrates the sensitive, selective, and specific detection of TM in 30 min. The performance of the sensor was confirmed using TM spiked chicken soup, resulting in a high percentage recovery (~97 ± 10%). The association of GO and labelled aptamer sensor platform has shown the rapid detection of TM in food, which is compared to other methods very sensitive, specific and performs in high throughput application.

Item ID: 63527
Item Type: Article (Research - C1)
ISSN: 1873-7072
Keywords: Aptasensor, Analytical assay, graphene oxide, Tropomyosin, Fluorescence assay, Shellfish-allergens, Allergen detection, Food safety
Copyright Information: © 2019 Elsevier Ltd. All rights reserved.
Date Deposited: 02 Jul 2020 02:18
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110701 Allergy @ 50%
10 TECHNOLOGY > 1004 Medical Biotechnology > 100402 Medical Biotechnology Diagnostics (incl Biosensors) @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 70%
92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920406 Food Safety @ 10%
92 HEALTH > 9202 Health and Support Services > 920203 Diagnostic Methods @ 20%
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