Diverse coral reef invertebrates exhibit patterns of phylosymbiosis

O'Brien, Paul A., Tan, Shangjin, Yang, Chentao, Frade, Pedro R., Andreakis, Nikolaos, Smith, Hillary A., Miller, David J., Webster, Nicole S., Zhang, Guojie, and Bourne, David G. (2020) Diverse coral reef invertebrates exhibit patterns of phylosymbiosis. ISME Journal: multidisciplinary journal of microbial ecology, 14. pp. 2211-2222.

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Microbiome assemblages of plants and animals often show a degree of correlation with host phylogeny; an eco-evolutionary pattern known as phylosymbiosis. Using 16S rRNA gene sequencing to profile the microbiome, paired with COI, 18S rRNA and ITS1 host phylogenies, phylosymbiosis was investigated in four groups of coral reef invertebrates (scleractinian corals, octocorals, sponges and ascidians). We tested three commonly used metrics to evaluate the extent of phylosymbiosis: (a) intraspecific versus interspecific microbiome variation, (b) topological comparisons between host phylogeny and hierarchical clustering (dendrogram) of host-associated microbial communities, and (c) correlation of host phylogenetic distance with microbial community dissimilarity. In all instances, intraspecific variation in microbiome composition was significantly lower than interspecific variation. Similarly, topological congruency between host phylogeny and the associated microbial dendrogram was more significant than would be expected by chance across all groups, except when using unweighted UniFrac distance (compared with weighted UniFrac and Bray-Curtis dissimilarity). Interestingly, all but the ascidians showed a significant positive correlation between host phylogenetic distance and associated microbial dissimilarity. Our findings provide new perspectives on the diverse nature of marine phylosymbioses and the complex roles of the microbiome in the evolution of marine invertebrates.

Item ID: 63428
Item Type: Article (Research - C1)
ISSN: 1751-7370
Copyright Information: © The Author(s), under exclusive licence to International Society for Microbial Ecology 2020
Additional Information:

All microbial data have been made available at the NCBI Sequence Read Archive under the BioProject accession number PRJNA577361 and host sequence data are available at the CNGB Sequence Archive under the accession numbers N_000000252.1–N_000000348.1

Funders: Beijing Genome Institute, Earthwatch Institute (EI), Mitsubishi Corporation, AIMS@JCU
Research Data: https://github.com/paobrien
Date Deposited: 10 Jun 2020 07:38
FoR Codes: 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310704 Microbial genetics @ 50%
31 BIOLOGICAL SCIENCES > 3104 Evolutionary biology > 310410 Phylogeny and comparative analysis @ 50%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%
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