Effect of bisphosphonates on the osteogenic activity of osteoprogenitor cells cultured on titanium surfaces

Sher, Judd, Miller, Catherine M., and Sharma, Dileep (2020) Effect of bisphosphonates on the osteogenic activity of osteoprogenitor cells cultured on titanium surfaces. International Journal of Oral & Maxillofacial Implants, 35 (5). pp. 939-947.

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DOI: 10.11607/jomi.8354

View at Publisher Website: https://doi.org/10.11607/jomi.8354

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Abstract

Purpose: This study investigated the effects of bisphosphonates (BPs) namely Alendronate (ALN) and Zoledronate (ZA) on the osteogenic activity of osteoprogenitor cells cultured on titanium surfaces at therapeutic doses in order to assess if altered osteoblastogenesis could compromise osseointegration and contribute in etiopathogenesis of painful disorders like BPrelated Osteonecrosis of the Jaw (BRONJ) following implant placement.

Materials and Methods: MC3T3-E1 Subclone 4 cells were utilised in this study. Therapeutic doses of ALN and ZA were calculated based off reported peak plasma concentrations. The viability, proliferation, adhesion, and mineralisation potential of cells was assessed using a LIVE/DEAD stain, alamarBlue® assay, immunofluorescence microscopy, and Alizarin Red S staining respectively.

Results: Therapeutic doses of ZA negatively affected cell viability, whereas therapeutic doses of ALN significantly enhanced cell differentiation and the amount of bone formation compared to the control. Conclusions: The findings of this study may provide some insight into the pathogenesis of BRONJ developing following implant placement in patients treated with ZA and may have promising implications towards improved wound healing and osseointegration in patients treated with ALN.


Item ID:	62954
Item Type:	Article (Research - C1)
ISSN:	1942-4434
Keywords:	Alendronate, Bisphosphonates, Dental Implants, Osseointegration, Osteoblasts, Zoledronate
Copyright Information:	© 2020 BY QUINTESSENCE PUBLISHING CO, INC. PRINTING OF THIS DOCUMENT IS RESTRICTED TO PERSONAL USE ONLY. NO PART MAY BE REPRODUCED OR TRANSMITTED IN ANY FORM WITHOUT WRITTEN PERMISSION FROM THE PUBLISHER. Authors can share their reviewed and accepted (postprint) manuscript via noncommercial platforms, such as their institutional repository, after a 12-month embargo period.
Funders:	Australian Dental Research Foundation (ADRF)
Date Deposited:	28 Jul 2020 23:34
FoR Codes:	32 BIOMEDICAL AND CLINICAL SCIENCES > 3203 Dentistry > 320302 Dental materials and equipment @ 40% 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310105 Cellular interactions (incl. adhesion, matrix, cell wall) @ 20% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3203 Dentistry > 320303 Dental therapeutics, pharmacology and toxicology @ 40%
SEO Codes:	92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920113 Oro-Dental Disorders @ 100%
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