Selecting extraction conditions for the production of liquid biostimulants from the freshwater macroalga Oedogonium intermedium

Neveux, Nicolas, Nugroho, A.A., Roberts, D.A., Vucko, M.J., and de Nys, R. (2020) Selecting extraction conditions for the production of liquid biostimulants from the freshwater macroalga Oedogonium intermedium. Journal of Applied Phycology. (In Press)

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Abstract

Liquid biostimulants made from macroalgae have traditionally been based on the processing of brown seaweeds collected from the wild. In this study, liquid extracts were produced from the freshwater green macroalga Oedogonium intermedium, cultivated in a land-based system. The first part of the study was aimed at selecting extraction conditions to maximize the liquid yield and the recovery of soluble solids from Oedogonium biomass, which resulted in the selection of a biomass-to-solvent ratio of 5% (w/v), an extraction time of 4 h, and a temperature of 70 °C. These extraction conditions were used in the second part of the study to assess the effect of extraction solvent, across the whole pH range, on root growth of tomato and mung bean seedlings. Root growth of tomato seedlings was highest for the acid extract prepared with 0.05 M HCl, and the alkali extract prepared with 0.01 M KOH. Therefore, these two extracts were further tested in a mung bean assay to determine their auxin-like activity, and were also analyzed to determine their elemental and hormone profiles. The selected acid and alkali extracts, at concentrations of 10% and 2–10% (v/v), respectively, had an auxin-like activity equivalent to 5 × 10−5 M indole-3-butyric acid, with the alkali extract having the highest concentration of auxins (120 ng IAA g−1 dry weight). This study highlights the potential of O. intermedium for the production of liquid extracts with biostimulant activity.

Item ID: 61159
Item Type: Article (Research - C1)
ISSN: 1573-5176
Keywords: macroalgae, plant biostimulant, fertilizer, hormone, auxin
Copyright Information: © 2019 Springer Nature Switzerland AG.
Date Deposited: 11 Dec 2019 05:14
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 50%
06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060701 Phycology (incl Marine Grasses) @ 50%
SEO Codes: 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8203 Industrial Crops > 820399 Industrial Crops not elsewhere classified @ 50%
86 MANUFACTURING > 8603 Processed Non-Food Agriculture Products (excl. Wood, Paper and Fibre) > 860302 Organic Fertilisers @ 50%
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