CD8aa intraepithelial lymphocytes arise from two main thymic precursors

Ruscher, Roland, Kummer, Rebecca L., Lee, You Jeong, Jameson, Stephen C., and Hogquist, Kristin A. (2017) CD8aa intraepithelial lymphocytes arise from two main thymic precursors. Nature Immunology, 18 (7). pp. 771-779.

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Abstract

TCRαβ+CD4−CD8α+CD8β− intestinal intraepithelial lymphocytes (CD8αα IELs) are an abundant population of thymus-derived T cells that protect the gut barrier surface. We sought to better define the thymic IEL precursor (IELp) through analysis of its maturation, localization and emigration. We defined two precursor populations among TCRβ+CD4−CD8− thymocytes by dependence on the kinase TAK1 and rigorous lineage-exclusion criteria. Those IELp populations included a nascent PD-1+ population and a T-bet+ population that accumulated with age. Both gave rise to intestinal CD8αα IELs after adoptive transfer. The PD-1+ IELp population included more strongly self-reactive clones and was largely restricted by classical major histocompatibility complex (MHC) molecules. Those cells localized to the cortex and efficiently emigrated in a manner dependent on the receptor S1PR1. The T-bet+ IELp population localized to the medulla, included cells restricted by non-classical MHC molecules and expressed the receptor NK1.1, the integrin CD103 and the chemokine receptor CXCR3. The two IELp populations further differed in their use of the T cell antigen receptor (TCR) α-chain variable region (Vα) and β-chain variable region (Vβ). These data provide a foundation for understanding the biology of CD8αα IELs.

Item ID: 60655
Item Type: Article (Research - C1)
ISSN: 1529-2916
Copyright Information: © 2017 Nature America,Inc.,part of Springer Nature. All rights reserved.
Funders: US National Institutes of Health (NIH)
Projects and Grants: NIH R37 AI39560, NIH PO1 AI35296, NIH K99 AI114889, NIH T35 AI118620-01
Date Deposited: 22 Oct 2019 01:51
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 50%
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