Tamoxifen affects the histology and hepatopancreatic lipid metabolism of swimming crab Portunus trituberculatus

Liu, Meimei, Feng, Qiangmei, Francis, David S., Turchini, Giovanni M., Zeng, Chaoshu, and Wu, Xugan (2019) Tamoxifen affects the histology and hepatopancreatic lipid metabolism of swimming crab Portunus trituberculatus. Aquatic Toxicology, 213. 105220.

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Abstract

Tamoxifen (TAM) is an antiestrogenic agent and can enter the aquatic environment in wastewater. It has been reported that TAM can induce hepatic steatosis in vertebrates, however, the effects of TAM exposure on lipid metabolism of hepatopancreas in crustaceans remains unclear. In this study, four TAM concentrations (0, 6.7, 13.4 and 20 mu g g(-1) crab body weight) were injected into the swimming-leg of swimming crabs Portunus trituberculatus, as a means of evaluating the effects of TAM on the expression levels of lipid metabolism-related genes, lipid composition, and hepatopancreas histology. The results showed that the mRNA levels of three lipogenic related genes (diacylglycerol acyltransferase 1 (DGAT1), acetyl-CoA carboxylase (ACC) and fatty acyl desaturase (FAD)) decreased significantly in the 6.7 mu g g(-1) and 20 mu g g(-1) TAM treatments compare to the control. The mRNA levels of fatty acid synthase (FAS) decreased significantly in a dose-dependent manner as TAM concentration increased. The mRNA levels of two lipid catabolism-related genes (acyl-CoA oxidase (ACOX) and fatty acid transport protein (FATP)) were down-regulated among the three TAM treatments, while the enzyme activity and mRNA level of carnitine palmitoyltransferase I (CPT-I) was up-regulated by TAM treatments. Compared to the control, the lowest levels of total lipids and phospholipids were detected in the 6.7 mu g g(-1) TAM treatment, while the 20 mu g g(-1) TAM treatment had the lowest free fatty acids concentration. The 6.7 mu g g(-1) TAM treatment had the lowest percentages of 16:1n-7, 18:1n-9, 18:1n-7 and total monounsaturated fatty acids (Sigma MDFA), whilst simultaneously recording the highest percentages of 18:2n-6 and 20:2n-6 in this treatment. Moreover, histological observations indicated that TAM caused the walls of the hepatopancreatic tubules to become brittle, with a concurrent increase in the number of blister-like cells. These results suggest that TAM damages the hepatopancreas and leads to a reduction in hepatopancreatic lipid deposition in P. trituberculatus.

Item ID: 59960
Item Type: Article (Research - C1)
ISSN: 1879-1514
Keywords: Portunus trituberculatus, Tamoxifen, Hepatopancreas, Lipid metabolism
Funders: Natural Science Foundation of China (NSFC), Shanghai Agriculture Committee (SAC)
Projects and Grants: NCFC No. 41276158, NCFC No. 41606169, SAC No. 2016-1-18
Date Deposited: 28 Aug 2019 07:31
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 60%
06 BIOLOGICAL SCIENCES > 0608 Zoology > 060808 Invertebrate Biology @ 40%
SEO Codes: 96 ENVIRONMENT > 9611 Physical and Chemical Conditions of Water > 961104 Physical and Chemical Conditions of Water in Marine Environments @ 30%
83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 70%
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