A rapid and inexpensive viability assay for zoospores and zoosporangia of Batrachochytrium dendrobatidis

Webb, Rebecca Jane, Berger, Lee, Skerratt, Lee F., and Roberts, Alexandra A. (2019) A rapid and inexpensive viability assay for zoospores and zoosporangia of Batrachochytrium dendrobatidis. Journal of Microbiological Methods. (In Press)

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Abstract

The fungus Batrachochytrium dendrobatidis is causing global amphibian declines. Here we describe a simple, rapid and inexpensive methylene blue staining protocol to determine B. dendrobatidis viability, regardless of life-stage. The viability of cells in suspension or adherent monolayers can be determined using either manual microscopy counting or colorimetric assay.

Item ID: 59882
Item Type: Article (Research - C1)
ISSN: 1872-8359
Keywords: Batrachochytrium dendrobatidis; Viability; Methylene blue; Zoosporangia
Copyright Information: © 2019 Published by Elsevier B.V.
Funders: Morris Animal Foundation (MAF), Holsworth Wildlife Research Endowment (HWRE)
Projects and Grants: MAF Pilot Grant D18ZO - 852
Date Deposited: 28 Aug 2019 02:36
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060505 Mycology @ 60%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 40%
SEO Codes: 96 ENVIRONMENT > 9604 Control of Pests, Diseases and Exotic Species > 960405 Control of Pests, Diseases and Exotic Species at Regional or Larger Scales @ 40%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 60%
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