A rapid and inexpensive viability assay for zoospores and zoosporangia of Batrachochytrium dendrobatidis
Webb, Rebecca Jane, Berger, Lee, Skerratt, Lee F., and Roberts, Alexandra A. (2019) A rapid and inexpensive viability assay for zoospores and zoosporangia of Batrachochytrium dendrobatidis. Journal of Microbiological Methods, 65. 105688.
|
PDF (Accepted Author Version)
- Accepted Version
Download (1MB) | Preview |
|
PDF (Published Version)
- Published Version
Restricted to Repository staff only |
View at Publisher Website: https://doi.org/10.1016/j.mimet.2019.105...
Abstract
The fungus Batrachochytrium dendrobatidis is causing global amphibian declines. Here we describe a simple, rapid and inexpensive methylene blue staining protocol to determine B. dendrobatidis viability, regardless of life-stage. The viability of cells in suspension or adherent monolayers can be determined using either manual microscopy counting or colorimetric assay.
Item ID: | 59882 |
---|---|
Item Type: | Article (Research - C1) |
ISSN: | 1872-8359 |
Keywords: | Batrachochytrium dendrobatidis; Viability; Methylene blue; Zoosporangia |
Related URLs: | |
Copyright Information: | © 2019 Published by Elsevier B.V. |
Funders: | Morris Animal Foundation (MAF), Holsworth Wildlife Research Endowment (HWRE) |
Projects and Grants: | MAF Pilot Grant D18ZO - 852 |
Date Deposited: | 28 Aug 2019 02:36 |
FoR Codes: | 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310705 Mycology @ 60% 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310702 Infectious agents @ 40% |
SEO Codes: | 96 ENVIRONMENT > 9604 Control of Pests, Diseases and Exotic Species > 960405 Control of Pests, Diseases and Exotic Species at Regional or Larger Scales @ 40% 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 60% |
Downloads: |
Total: 1077 Last 12 Months: 24 |
More Statistics |