A rapid and inexpensive viability assay for zoospores and zoosporangia of Batrachochytrium dendrobatidis

Webb, Rebecca Jane, Berger, Lee, Skerratt, Lee F., and Roberts, Alexandra A. (2019) A rapid and inexpensive viability assay for zoospores and zoosporangia of Batrachochytrium dendrobatidis. Journal of Microbiological Methods, 65. 105688.

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DOI: 10.1016/j.mimet.2019.105688

View at Publisher Website: https://doi.org/10.1016/j.mimet.2019.105...

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Abstract

The fungus Batrachochytrium dendrobatidis is causing global amphibian declines. Here we describe a simple, rapid and inexpensive methylene blue staining protocol to determine B. dendrobatidis viability, regardless of life-stage. The viability of cells in suspension or adherent monolayers can be determined using either manual microscopy counting or colorimetric assay.


Item ID:	59882
Item Type:	Article (Research - C1)
ISSN:	1872-8359
Keywords:	Batrachochytrium dendrobatidis; Viability; Methylene blue; Zoosporangia
Related URLs:	https://researchonline.jcu.edu.au/80683/
Copyright Information:	© 2019 Published by Elsevier B.V.
Funders:	Morris Animal Foundation (MAF), Holsworth Wildlife Research Endowment (HWRE)
Projects and Grants:	MAF Pilot Grant D18ZO - 852
Date Deposited:	28 Aug 2019 02:36
FoR Codes:	31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310705 Mycology @ 60% 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310702 Infectious agents @ 40%
SEO Codes:	96 ENVIRONMENT > 9604 Control of Pests, Diseases and Exotic Species > 960405 Control of Pests, Diseases and Exotic Species at Regional or Larger Scales @ 40% 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 60%
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