Multiple response optimisation of the aqueous extraction of high quality ulvan from Ulva ohnoi

Glasson, Christopher R.K., Donnet, Luke, Angell, Alex, Vucko, Matthew J., Lorbeer, Andrew J., Vamvounis, George, de Nys, Rocky, and Magnusson, Marie (2019) Multiple response optimisation of the aqueous extraction of high quality ulvan from Ulva ohnoi. Bioresource Technology Reports, 7. 100262.

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Abstract

Response surface methodology was used to determine the effects of the solvent pH, the temperature of extraction, and the duration of extraction on the yield, purity, molecular weight, viscosity, and total metal content of ulvan extracted from U. ohnoi. Quadratic models identified the optimised responses for yield (72.6%) purity (68.2% w/w), molecular weight (92.9 kDa), viscosity (491.1 s), and total metal content (~0 mg/kg). These responses occurred between a solvent pH of 2.2–4.0, an extraction temperature of 61.3–90.0 °C, and an extraction duration of 55.0–90.0 min. The overall desirability of the ulvan product was determined using a Derringer's desirability function, which identified a solvent pH of 2.92, an extraction temperature of 90 °C, and an extraction duration of 90 min. These extraction conditions minimise the requirement for downstream purification and are suitable for upscaling the extraction of a high quality ulvan product.

Item ID: 59094
Item Type: Article (Research - C1)
ISSN: 2589-014X
Keywords: Polysaccharide; Ulvan; Biorefinery; Optimization; Seaweed
Date Deposited: 14 Aug 2019 23:15
FoR Codes: 10 TECHNOLOGY > 1003 Industrial Biotechnology > 100302 Bioprocessing, Bioproduction and Bioproducts @ 40%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 60%
SEO Codes: 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8203 Industrial Crops > 820399 Industrial Crops not elsewhere classified @ 100%
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