Parentage determination of the mud crab Scylla paramamosain using microsatellite markers

Xu, Xiaojun, Wang, Guizhong, Zeng, Chaoshu, and Li, Shaojing (2018) Parentage determination of the mud crab Scylla paramamosain using microsatellite markers. Aquaculture Research, 49 (1). pp. 217-221.

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Abstract

This study evaluated the properties of nine Scylla paramamosain microsatellite loci screened by us previously for inclusion in a parentage assignment marker suite. These nine highly polymorphic markers (mean He=0.847 and PIC=0.830) were determined as being suitable for parentage assignment. Simulations based on allele frequency data from 15 known maternal families (165 individuals) demonstrated that at least four loci were required to assign >95% of offspring to maternal parents with 95% confidence. In actual parentage assignments, all progenies were assigned to the maternal parents with six or more loci, which was similar to the simulation predictions. Our results suggest that this set of microsatellites provide a powerful and efficient tool for identifying pedigree information for selective breeding programmes of S. paramamosain.

Item ID: 57447
Item Type: Article (Research - C1)
ISSN: 1365-2109
Keywords: microsatellite; offspring; parentage determination; Scylla paramamosain
Copyright Information: © 2017 John Wiley & Sons Ltd
Funders: National Science Foundation of China (NSFC)
Projects and Grants: NSFC Grant Number 31472294
Date Deposited: 19 Mar 2019 05:17
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 100%
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