Differences in diet and biotransformation enzymes of coral reef butterflyfishes between Australia and Hawaii

Maldonado, Aileen, Nowicki, Jessica, Pratchett, Morgan S., and Schlenk, Daniel (2019) Differences in diet and biotransformation enzymes of coral reef butterflyfishes between Australia and Hawaii. Comparative Biochemistry and Physiology Part C: toxicology & pharmacology, 216. pp. 1-9.

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Abstract

Many reef fishes are capable of feeding on chemically-defended benthic prey, such as soft (alcyonarian) corals; however, little is known about the molecular mechanisms that underpin allelochemical biotransformation and detoxification. Butterflyfishes (Chaetodon: Chaetdontidae) are a useful group for comparatively exploring links between biotransformation enzymes and diet, because they commonly feed on chemically defended prey. Moreover, diets of some species vary among geographic locations. This study compares gene expression, protein and enzymatic activity of key detoxification enzymes (cytochrome P450 (CYP) 2, 3, epoxide hydrolase, glutathione transferase and UDP-glucuronosyltransferase) in livers of four coral-feeding butterflyfish species between Australia and Hawaii, where these fishes differ in diet composition. For C. kleinii, C. auriga, and C. unimaculatus, we found higher CYP2 and CYP3 levels were linked to more allelochemically rich diets in Australia relative to Hawaii. For C. lunulatus from Hawaii CYP2 and CYP3 levels were 1 to 20-fold higher than C. lunulatus from Australia, possibly due to their predominant prey in Hawaii (Porities spp.) being richer in allelochemicals. UGT, GST and epoxide hydrolase varied between species and location and did not correspond to any specific dietary preference or location. Higher levels of CYP2 and CYP3A isozymes in species that feed on allelochemically-rich prey suggest that these biotransformation enzymes may be involved in detoxification of coral dietary allelochemicals in butterflyfishes.

Item ID: 57175
Item Type: Article (Research - C1)
ISSN: 1878-1659
Keywords: butterflyfish, coral, corallivores, detoxification, feeding strategies, toxin
Copyright Information: © 2018 Elsevier Inc. All rights reserved.
Date Deposited: 20 Feb 2019 07:49
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 100%
SEO Codes: 96 ENVIRONMENT > 9605 Ecosystem Assessment and Management > 960507 Ecosystem Assessment and Management of Marine Environments @ 100%
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